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Literature DB >> 6290454

Molecular cloning of the spo0B sporulation locus in bacteriophage lambda.

F A Ferrari, D Lang, E Ferrari, J A Hoch.

Abstract

The stage 0 sporulation locus spo0B has been mapped by transformation between the pheA and spoIVF loci. Analysis of the behavior of alleles of the spo0B locus in trpE26 merodiploid strains indicates that all of the known alleles of this locus comprise a single complementation group. The spoIVF88 mutation was found to reside in a separate complementation group. The chromosomal region surrounding and including the spo0B locus was cloned in the lambda vector Charon 4A. Extensive restriction endonuclease analyses of the inserts in these phage revealed that an EcoRI fragment of DNA of 2.3 kilobases had transforming activity for spo0B mutations. Examination of the physical and genetic maps of the locus suggested that the entire spo0B locus is contained within this fragment. Subcloning of restriction endonuclease fragments of the lambda inserts and transformation analyses allowed assignment of surrounding genetic loci to specific DNA fragments.

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Year: 1982 PMID： 6290454 PMCID： PMC221534 DOI： 10.1128/jb.152.2.809-814.1982

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

18 in total

1. REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.

Authors: C Anagnostopoulos; J Spizizen
Journal: J Bacteriol Date: 1961-05 Impact factor: 3.490

2. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors: P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal: J Mol Biol Date: 1977-06-15 Impact factor: 5.469

3. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.

Authors: M Dagert; S D Ehrlich
Journal: Gene Date: 1979-05 Impact factor: 3.688

4. Molecular cloning of the DNA ligase gene from bacteriophage T4. II. Amplification and preparation of the gene product.

Authors: N E Murray; S A Bruce; K Murray
Journal: J Mol Biol Date: 1979-08-15 Impact factor: 5.469

5. Chromosomal location of pleiotropic negative sporulation mutations in Bacillus subtilis.

Authors: J A Hoch; J L Mathews
Journal: Genetics Date: 1973-02 Impact factor: 4.562

Review 6. The Bacillus subtilis chromosome.

Authors: D J Henner; J A Hoch
Journal: Microbiol Rev Date: 1980-03

7. The isolation of structural genes from libraries of eucaryotic DNA.

Authors: T Maniatis; R C Hardison; E Lacy; J Lauer; C O'Connell; D Quon; G K Sim; A Efstratiadis
Journal: Cell Date: 1978-10 Impact factor: 41.582

8. Chromosomal location and properties of radiation sensitivity mutations in Bacillus subtilis.

Authors: J A Hoch; C Anagnostopoulos
Journal: J Bacteriol Date: 1970-08 Impact factor: 3.490

9. Developmentally regulated transcription in a cloned segment of the Bacillus subtilis chromosome.

Authors: J F Ollington; W G Haldenwang; T V Huynh; R Losick
Journal: J Bacteriol Date: 1981-08 Impact factor: 3.490

10. Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325.

Authors: X Soberon; L Covarrubias; F Bolivar
Journal: Gene Date: 1980-05 Impact factor: 3.688

12 in total

Review 1. Occurrence, recognition, and reversion of spontaneous, sporulation-deficient Bacillus anthracis mutants that arise during laboratory culture.

Authors: Inka Sastalla; Stephen H Leppla
Journal: Microbes Infect Date: 2011-11-28 Impact factor: 2.700

10. The divIVB region of the Bacillus subtilis chromosome encodes homologs of Escherichia coli septum placement (minCD) and cell shape (mreBCD) determinants.

Authors: A W Varley; G C Stewart
Journal: J Bacteriol Date: 1992-11 Impact factor: 3.490

Molecular cloning of the spo0B sporulation locus in bacteriophage lambda.

1. REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS.

2. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

3. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.

4. Molecular cloning of the DNA ligase gene from bacteriophage T4. II. Amplification and preparation of the gene product.

5. Chromosomal location of pleiotropic negative sporulation mutations in Bacillus subtilis.

Review 6. The Bacillus subtilis chromosome.

7. The isolation of structural genes from libraries of eucaryotic DNA.

8. Chromosomal location and properties of radiation sensitivity mutations in Bacillus subtilis.

9. Developmentally regulated transcription in a cloned segment of the Bacillus subtilis chromosome.

10. Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325.

Review 1. Occurrence, recognition, and reversion of spontaneous, sporulation-deficient Bacillus anthracis mutants that arise during laboratory culture.

2. Cloning, characterization, and expression of the spoVB gene of Bacillus subtilis.

3. The Bacillus subtilis spo0B stage 0 sporulation operon encodes an essential GTP-binding protein.

4. The effect of spo0 mutations on the expression of spo0A- and spo0F-lacZ fusions.

5. Transcriptional regulation and structure of the Bacillus subtilis sporulation locus spoIIIC.

6. The complete DNA sequence and regulatory regions of the Bacillus licheniformis spoOH gene.

7. Cloning of sporulation genes spo0A and spo0C of Bacillus subtilis onto rho 11 temperate bacteriophage.

8. Sequence analysis of the spo0B locus reveals a polycistronic transcription unit.

9. Nucleotide sequence of the spo0B gene of Bacillus subtilis and regulation of its expression.

10. The divIVB region of the Bacillus subtilis chromosome encodes homologs of Escherichia coli septum placement (minCD) and cell shape (mreBCD) determinants.