| Literature DB >> 6762478 |
A Trevisan, F Gudat, C Busachi, E Stöcklin, L Bianchi.
Abstract
Factors influencing the preservation of HBcAg in formalin-fixed, paraffin-embedded liver tissue have been analysed. Fixation time, choice of the embedding medium, deparaffinization and selection of anti-HBc proved to be critical variables. For optimal results, fixation in 4% buffered formalin for no longer than 24-48 h, the use of wax with a low melting point, a heating step prior to deparaffinization, and a search for anti-HBc specifically reactive for paraffinated HBcAg are recommended.Entities:
Mesh：
Substances：
Year: 1982 PMID： 6762478 DOI： 10.1111/j.1600-0676.1982.tb00831.x
Source DB: PubMed Journal: Liver ISSN： 0106-9543