Literature DB >> 1752980

Rapid detection of hepatitis B virus DNA in liver tissue by in situ hybridisation and its combination with immunohistochemistry for simultaneous detection of HBV antigens.

J Y Lau1, N V Naoumov, G J Alexander, R Williams.   

Abstract

A rapid technique using a non-radioactive receptor molecule (digoxigenin) for intrahepatic hepatitis B virus (HBV) DNA detection using in situ hybridisation was developed. It can be adapted for use in combination with standard immunohistochemistry for simultaneous detection of both HBV DNA and HBV antigens. The total time required for dual detection of HBV antigens and HBV DNA starting from paraffin wax liver sections was two working days. A good signal to background ratio for the detection of HBV DNA was always obtained using this labelling. This technique is cheap, safe, and relatively simple which makes it an ideal tool for the detection of intrahepatic HBV DNA for both routine diagnostic purposes and in research.

Entities:  

Mesh：

Substances：

Year:  1991        PMID： 1752980      PMCID： PMC496627          DOI： 10.1136/jcp.44.11.905

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN： 0021-9746            Impact factor:   3.411


  10 in total

1.  Combined use of in situ hybridization and unlabeled antibody peroxidase anti-peroxidase methods: simultaneous detection of type I procollagen mRNAs and factor VIII-related antigen epitopes in keloid tissue.

Authors:  S Sollberg; J Peltonen; J Uitto
Journal:  Lab Invest       Date:  1991-01       Impact factor: 5.662

2.  Detection of HBV-DNA using a digoxigenin-labelled probe. A rapid technique without loss of sensitivity.

Authors:  N V Naoumov; J Y Lau; H M Daniels; G J Alexander; R Williams
Journal:  J Hepatol       Date:  1991-05       Impact factor: 25.083

3.  In situ hybridization with digoxigenin-labeled DNA of human papillomaviruses (HPV 16/18) in HeLa and SiHa cells.

Authors:  H B Heiles; E Genersch; C Kessler; R Neumann; H J Eggers
Journal:  Biotechniques       Date:  1988 Nov-Dec       Impact factor: 1.993

4.  Widespread presence of cytoplasmic HBcAg in hepatitis B infected liver detected by improved immunochemical methods.

Authors:  E J Gowans; C J Burrell
Journal:  J Clin Pathol       Date:  1985-04       Impact factor: 3.411

5.  Expression of the hepatitis B virus surface, core and E antigen genes by stable rat and mouse cell lines.

Authors:  N M Gough; K Murray
Journal:  J Mol Biol       Date:  1982-11-25       Impact factor: 5.469

6.  An improved method for HBcAg demonstration in paraffin-embedded liver tissue.

Authors:  A Trevisan; F Gudat; C Busachi; E Stöcklin; L Bianchi
Journal:  Liver       Date:  1982-12

7.  In situ hybridisation with digoxigenin-labelled DNA probes for detection of viral genomes.

Authors:  Y Furuta; T Shinohara; K Sano; M Meguro; K Nagashima
Journal:  J Clin Pathol       Date:  1990-10       Impact factor: 3.411

8.  In situ hybridisation in formalin fixed, paraffin wax embedded liver specimens: method for detecting human and viral DNA using biotinylated probes.

Authors:  N V Naoumov; G J Alexander; A L Eddleston; R Williams
Journal:  J Clin Pathol       Date:  1988-07       Impact factor: 3.411

9.  Interphase cytogenetics using biotin and digoxigenin labelled probes I: relative sensitivity of both reporter molecules for detection of HPV16 in CaSki cells.

Authors:  C S Herrington; J Burns; A K Graham; M Evans; J O McGee
Journal:  J Clin Pathol       Date:  1989-06       Impact factor: 3.411

10.  Interphase cytogenetics using biotin and digoxigenin labelled probes II: Simultaneous differential detection of human and papilloma virus nucleic acids in individual nuclei.

Authors:  C S Herrington; J Burns; A K Graham; B Bhatt; J O McGee
Journal:  J Clin Pathol       Date:  1989-06       Impact factor: 3.411
  10 in total
  5 in total

1.  In situ analysis of intrahepatic virological events in chronic hepatitis B virus infection.

Authors:  Xiaonan Zhang; Wei Lu; Ye Zheng; Weixia Wang; Lu Bai; Liang Chen; Yanling Feng; Zhanqing Zhang; Zhenghong Yuan
Journal:  J Clin Invest       Date:  2016-02-22       Impact factor: 14.808

2.  Hepatic HLA antigen display in chronic hepatitis B virus infection. Relation to hepatic expression of HBV genome/gene products and liver histology.

Authors:  J Y Lau; G L Bird; N V Naoumov; R Williams
Journal:  Dig Dis Sci       Date:  1993-05       Impact factor: 3.199

3.  Hepatic expression of interferon-alpha in chronic hepatitis B virus infection.

Authors:  J W Fang; P C Wu; C L Lai; C K Lo; A Meager; J Y Lau
Journal:  Dig Dis Sci       Date:  1994-09       Impact factor: 3.199

4.  Single Hepatocyte Hepatitis B Virus Transcriptional Landscape in HIV Coinfection.

Authors:  Ashwin Balagopal; Hyon S Hwang; Tanner Grudda; Jeffrey Quinn; Richard K Sterling; Mark S Sulkowski; Chloe L Thio
Journal:  J Infect Dis       Date:  2020-04-07       Impact factor: 5.226

Review 5.  Transaminase Elevations during Treatment of Chronic Hepatitis B Infection: Safety Considerations and Role in Achieving Functional Cure.

Authors:  Andrew Vaillant
Journal:  Viruses       Date:  2021-04-23       Impact factor: 5.048
  5 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.