Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 IncD, a genetic locus in F responsible for incompatibility with several plasmids of the IncFI group.

Literature DB >> 6759876

IncD, a genetic locus in F responsible for incompatibility with several plasmids of the IncFI group.

R C Gardner, L Malcolm, P L Bergquist, H E Lane.

Abstract

Cloning of mini-F DNA segments has led to the identification and mapping of a locus, incD, involved in incompatibility reactions with many IncFI plasmids. The cloned incD locus expressed incompatibility with F, R386, and six other IncFI plasmids but not with ColV3-K30 or pHH507 which lack sequence homology with the incD region. A sequence of 360 bp (48.66-49.02 FKB) was found to be sufficient for expression of incD incompatibility. Multicopy vectors containing incD are compatible with each other, but can be displaced by mini-F plasmids deleted for incD. These results indicate that incD-mediated incompatibility reactions require the presence of replication genes to which incD is normally linked. The degree of incompatibility exercised by incD is moderate compared with that of other inc loci in F, suggesting that incD is involved in an aspect of plasmid maintenance, such as partition, different from the functions of the other inc loci.

Mesh：

Substances：
DNA, Bacterial

Year: 1982 PMID： 6759876 DOI： 10.1007/bf00332699

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

26 in total

1. Partitioning of bacterial plasmids during cell division: a cis-acting locus that accomplishes stable plasmid inheritance.

Authors: P A Meacock; S N Cohen
Journal: Cell Date: 1980-06 Impact factor: 41.582

IncD, a genetic locus in F responsible for incompatibility with several plasmids of the IncFI group.

1. Partitioning of bacterial plasmids during cell division: a cis-acting locus that accomplishes stable plasmid inheritance.

2. Essential regions for replication of a stringent and a relaxed plasmid in Escherichia coli.

3. Second EcoRI fragment of F capable of self-replication.

4. Dislodgement and thymineless elimination of N-group plasmids.

5. Mapping of the drug resistance genes carried by the r-determinant of the R100.1 plasmid.

6. Analysis of transcripts from plasmid mini-F by electron microscopy of R loops.

7. Effects of Tn5 insertion in the incD region on mini-F maintenance and polypeptide synthesis.

8. Molecular homology and incompatibility in the IncFI plasmid Group.

9. A proposed system for nomenclature for incompatibility genes of the Escherichia coli sex factor, plasmid F.

10. Plasmids useable as gene-cloning vectors in an in vitro packaging by coliphage lambda: "cosmids".

1. Structural elements required for replication and incompatibility of the Rhizobium etli symbiotic plasmid.

2. Analysis of the F plasmid centromere.

3. Partitioning of the F plasmid: overproduction of an essential protein for partition inhibits plasmid maintenance.

4. D protein of miniF plasmid acts as a repressor of transcription and as a site-specific resolvase.

5. SOS induction by thermosensitive replication mutants of miniF plasmid.

6. Twelve 43-base-pair repeats map in a cis-acting region essential for partition of plasmid mini-F.

7. Mini-F protein that binds to a unique region for partition of mini-F plasmid DNA.

8. Cloning and analysis of pif, replication and leading regions of the F plasmid.

9. Effects of Tn5 insertion in the incD region on mini-F maintenance and polypeptide synthesis.

10. Enteropathogenic Escherichia coli: identification of a gene cluster coding for bundle-forming pilus morphogenesis.