Cytochemical reactivity of T mu lymphocytes in human lymphatic tissue for dipeptidylaminopeptidase IV.

The enzyme dipeptidylaminopeptidase IV (DAP IV; EC 3.4.14.-) was recently shown cytochemically to be confined, in blood and bone marrow, to human T cells bearing Fc receptors for IgM (T mu lymphocytes). This observation, confirmed by direct biochemical tests, stimulated us to study the histochemical distribution of DAP IV in normal human lymphatic tissue. In cryostat sections of lymph node, tonsil and thymus, DAP IV was detectable only in lymphocytes, Hassal's corpuscles and the endothelia of vessels. The distribution pattern of DAP IV-positive lymphocytes accorded well with results obtained with human T cell antisera. Compared to cytochemical reactions for other enzymes, such as acid esterase, DAP IV has the advantage that it does not stain monocytes, B lymphocytes or other mononuclear cells. Further, it does not depend on a particular type of staining pattern like, for example, the dot-like reaction product of acid esterase in T lymphocytes. Since the reaction for DAP IV remains more or less unchanged in month-old sections, it is easily adaptable to routine work and has the potentiality of being applied to the diagnosis of T cell lymphomas.