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Literature DB >> 6752207

Improved double immunoenzyme labeling using alkaline phosphatase and horseradish peroxidase.

Abstract

Alkaline phosphatase and peroxidase complexes were applied to tissue sections simultaneously to label two antigens. An azo-coupling method was used to demonstrate the alkaline phosphatase activity. The final result gave clear contrast between the two antigens and allowed the use of haematoxylin as the nuclear counterstain.

Entities: Chemical Species

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Year: 1982 PMID： 6752207 PMCID： PMC497889 DOI： 10.1136/jcp.35.10.1092

Source DB: PubMed Journal: J Clin Pathol ISSN： 0021-9746 Impact factor: 3.411

5 in total

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Authors: E Heyderman
Journal: J Clin Pathol Date: 1979-10 Impact factor: 3.411

2. The unlabeled antibody enzyme method of immunohistochemistry: preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes.

Authors: L A Sternberger; P H Hardy; J J Cuculis; H G Meyer
Journal: J Histochem Cytochem Date: 1970-05 Impact factor: 2.479

3. Alkaline phosphatase and peroxidase for double immunoenzymatic labelling of cellular constituents.

Authors: D Y Mason; R Sammons
Journal: J Clin Pathol Date: 1978-05 Impact factor: 3.411

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Authors: H J Stutte
Journal: Histochemie Date: 1967

5. An immunohistological study of human lymphoma.

Authors: D Y Mason; J I Bell; B Christensson; P Biberfeld
Journal: Clin Exp Immunol Date: 1980-05 Impact factor: 4.330

5 in total

13 in total

1. Multiple immunoenzyme staining: methods and visualizations for the observation with spectral imaging.

Authors: Chris M van der Loos
Journal: J Histochem Cytochem Date: 2007-12-23 Impact factor: 2.479

Review 2. Practical suggestions for successful immunoenzyme double-staining experiments.

Authors: C M van der Loos; A E Becker; J J van den Oord
Journal: Histochem J Date: 1993-01

Review 3. Cytochemical detection systems for in situ hybridization, and the combination with immunocytochemistry, 'who is still afraid of red, green and blue?'.

Authors: E J Speel; F C Ramaekers; A H Hopman
Journal: Histochem J Date: 1995-11

4. Differential expression of MHC- and macrophage-associated antigens in human fetal and postnatal small intestine.

Authors: J Harvey; D B Jones; D H Wright
Journal: Immunology Date: 1990-03 Impact factor: 7.397

5. Double immunoenzymatic labeling of lymphomatous tissues for both immunologic phenotype and a malignancy-associated nucleolar antigen.

Authors: M Lu; F M Davis; R J Ford
Journal: Am J Pathol Date: 1986-04 Impact factor: 4.307

6. Circadian rhythms in the number of gastrin cells, DNA synthesizing cells and labelling index of gastrin cells in the antral mucosa of the rat.

Authors: Y Fukuchi
Journal: Gastroenterol Jpn Date: 1990-02

7. Alzheimer's disease beta-amyloid peptides are released in association with exosomes.

Authors: Lawrence Rajendran; Masanori Honsho; Tobias R Zahn; Patrick Keller; Kathrin D Geiger; Paul Verkade; Kai Simons
Journal: Proc Natl Acad Sci U S A Date: 2006-07-12 Impact factor: 11.205

8. CGRP-immunoreactive endocrine cell proliferation in normal and hypoxic rat lung studied by immunocytochemical detection of incorporation of 5'-bromodeoxyuridine.

Authors: L M Montuenga; D R Springall; J Gaer; F J Winter; L Zhao; J T McBride; K M Taylor; G Barer; J M Polak
Journal: Cell Tissue Res Date: 1992-04 Impact factor: 5.249

9. Sensitivity of various visualization methods for peroxidase and alkaline phosphatase activity in immunoenzyme histochemistry.

Authors: A S De Jong; M Van Kessel-van Vark; A K Raap
Journal: Histochem J Date: 1985-10

10. Distribution of LCA protein subspecies and the cellular adhesion molecules LFA-1, ICAM-1 and p150,95 within human foetal thymus.

Authors: J E Harvey; D B Jones
Journal: Immunology Date: 1990-06 Impact factor: 7.397