Literature DB >> 6749496

Cloning and amplified expression of the tyrosyl-tRNA synthetase genes of Bacillus stearothermophilus and Escherichia coli.

D G Barker.   

Abstract

Two fragments of DNA which carry the genes coding for the tyrosyl-tRNA synthetases of Escherichia coli and Bacillus stearothermophilus have been cloned into the plasmid pBR322 and were selected by complementation of an E. coli temperature-sensitive mutant. Transformation of this strain with either of the recombinant plasmids results in a 100-fold increase in tyrosyl-tRNA synthetase activity measured in vitro and the protein products co-migrate with the corresponding purified enzymes on polyacrylamide gels.

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Year:  1982        PMID： 6749496     DOI： 10.1111/j.1432-1033.1982.tb06691.x

Source DB:  PubMed          Journal:  Eur J Biochem        ISSN： 0014-2956


  10 in total

1.  Construction of heterodimer tyrosyl-tRNA synthetase shows tRNATyr interacts with both subunits.

Authors:  P Carter; H Bedouelle; G Winter
Journal:  Proc Natl Acad Sci U S A       Date:  1986-03       Impact factor: 11.205

Review 2.  Biocatalysis made to order.

Authors:  G Tripathi
Journal:  Appl Biochem Biotechnol       Date:  1988-10       Impact factor: 2.926

3.  Overproduction of tyrosyl-tRNA synthetase is toxic to Escherichia coli: a genetic analysis.

Authors:  H Bedouelle; V Guez; A Vidal-Cros; M Hermann
Journal:  J Bacteriol       Date:  1990-07       Impact factor: 3.490

4.  Response of a phage modification factor to enhanced production of its target molecule.

Authors:  N J Olson; G L Marchin
Journal:  J Virol       Date:  1985-02       Impact factor: 5.103

5.  Thioredoxin from Bacillus acidocaldarius: characterization, high-level expression in Escherichia coli and molecular modelling.

Authors:  S Bartolucci; A Guagliardi; E Pedone; D De Pascale; R Cannio; L Camardella; M Rossi; G Nicastro; C de Chiara; P Facci; G Mascetti; C Nicolini
Journal:  Biochem J       Date:  1997-11-15       Impact factor: 3.857

6.  Competition of aminoacyl-tRNA synthetases for tRNA ensures the accuracy of aminoacylation.

Authors:  J M Sherman; M J Rogers; D Söll
Journal:  Nucleic Acids Res       Date:  1992-04-11       Impact factor: 16.971

7.  Competition of aminoacyl-tRNA synthetases for tRNA ensures the accuracy of aminoacylation.

Authors:  J M Sherman; M J Rogers; D Söll
Journal:  Nucleic Acids Res       Date:  1992-06-11       Impact factor: 16.971

8.  Deletion mutagenesis using an 'M13 splint': the N-terminal structural domain of tyrosyl-tRNA synthetase (B. stearothermophilus) catalyses the formation of tyrosyl adenylate.

Authors:  M M Waye; G Winter; A J Wilkinson; A R Fersht
Journal:  EMBO J       Date:  1983       Impact factor: 11.598

9.  Cloning and expression of the fbc operon encoding the FeS protein, cytochrome b and cytochrome c1 from the Rhodopseudomonas sphaeroides b/c1 complex.

Authors:  N Gabellini; U Harnisch; J E McCarthy; G Hauska; W Sebald
Journal:  EMBO J       Date:  1985-02       Impact factor: 11.598

10.  Clamp-loader-helicase interaction in Bacillus. Leucine 381 is critical for pentamerization and helicase binding of the Bacillus tau protein.

Authors:  A Haroniti; R Till; M C M Smith; P Soultanas
Journal:  Biochemistry       Date:  2003-09-23       Impact factor: 3.162
  10 in total

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