Literature DB >> 6743336

Alkaline transfer of DNA to plastic membrane.

P Chomczynski, P K Qasba.   

Abstract

DNA forms a stable complex with Gene Screen Plus membrane at alkaline pH. Based on this, a method of alkaline transfer of DNA from agarose gel to Gene Screen Plus membrane was elaborated. The procedure entails the use of 0.4 M NaOH for both, the DNA denaturation and DNA transfer steps. The alkaline transfer offers a higher hybridization efficiency and simplifies the transfer procedure as compared with the standard method of DNA transfer at neutral pH. In addition, it can be used to remove RNA contamination from the transferred DNA.

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Year:  1984        PMID： 6743336     DOI： 10.1016/0006-291x(84)90480-7

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  102 in total

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Journal:  Plant Mol Biol       Date:  1990-04       Impact factor: 4.076

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Journal:  Nucleic Acids Res       Date:  1989-07-11       Impact factor: 16.971

6.  Upstream activation sequence-dependent alteration of chromatin structure and transcription activation of the yeast GAL1-GAL10 genes.

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Journal:  Mol Cell Biol       Date:  1989-04       Impact factor: 4.272

7.  Bacillus subtilis generates a major specific deletion in pAM beta 1.

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Journal:  Appl Environ Microbiol       Date:  1987-10       Impact factor: 4.792

8.  Tripeptidase gene (pepT) of Lactococcus lactis: molecular cloning and nucleotide sequencing of pepT and construction of a chromosomal deletion mutant.

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Journal:  J Bacteriol       Date:  1994-05       Impact factor: 3.490

9.  The primary structure of sensory rhodopsin II: a member of an additional retinal protein subgroup is coexpressed with its transducer, the halobacterial transducer of rhodopsin II.

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Journal:  J Bacteriol       Date:  1991-01       Impact factor: 3.490
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