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Literature DB >> 16896525

Characterisation of the novel restriction endonuclease SuiI from Sulfolobus islandicus.

Stefan Söllner¹, Silvia Berkner, Georg Lipps.

Abstract

A restriction endonuclease activity from Sulfolobus islandicus REN2H1 was purified by phosphocellulose and cation exchange chromatography. The enzyme cuts DNA at the recognition site GCwGC as could be shown by restriction analysis of plasmids and short synthetic duplex DNA. The cleavage occurs after the first guanosine base and is inhibited by 5-methyl-cytosine methylation. The restriction activity is salt-sensitive and has an optimal activity around 70 degrees C.

Entities: Chemical Species

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Year: 2006 PMID： 16896525 DOI： 10.1007/s00792-006-0019-0

Source DB: PubMed Journal: Extremophiles ISSN： 1431-0651 Impact factor: 2.395

11 in total

1. Modular organization of related Archaeal plasmids encoding different restriction-modification systems in Methanobacterium thermoformicicum.

Authors: J Nölling; F J van Eeden; R I Eggen; W M de Vos
Journal: Nucleic Acids Res Date: 1992-12-25 Impact factor: 16.971

2. PspGI, a type II restriction endonuclease from the extreme thermophile Pyrococcus sp.: structural and functional studies to investigate an evolutionary relationship with several mesophilic restriction enzymes.

Authors: Vera Pingoud; Charlotte Conzelmann; Steffen Kinzebach; Anna Sudina; Valeri Metelev; Elena Kubareva; Janusz M Bujnicki; Rudi Lurz; Gerhild Lüder; Shuang-Yong Xu; Alfred Pingoud
Journal: J Mol Biol Date: 2003-06-20 Impact factor: 5.469

3. Complete nucleotide sequence of the Sulfolobus islandicus multicopy plasmid pRN1.

Authors: P J Keeling; H P Klenk; R K Singh; O Feeley; C Schleper; W Zillig; W F Doolittle; C W Sensen
Journal: Plasmid Date: 1996-03 Impact factor: 3.466

4. Phenotypic characterization of the archaebacterial genus Sulfolobus: comparison of five wild-type strains.

Authors: D W Grogan
Journal: J Bacteriol Date: 1989-12 Impact factor: 3.490

5. Cytosine methylation by the SuaI restriction-modification system: implications for genetic fidelity in a hyperthermophilic archaeon.

Authors: Dennis W Grogan
Journal: J Bacteriol Date: 2003-08 Impact factor: 3.490

6. A restriction enzyme Tha I from the thermophilic mycoplasma Thermoplasma acidophilum.

Authors: D J McConnell; D G Searcy; J G Sutcliffe
Journal: Nucleic Acids Res Date: 1978-06 Impact factor: 16.971

7. A restriction endonuclease SuaI from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius.

Authors: D A Prangishvili; R P Vashakidze; M G Chelidze
Journal: FEBS Lett Date: 1985-11-11 Impact factor: 4.124

8. Characterization of an extremely thermostable restriction enzyme, PspGI, from a Pyrococcus strain and cloning of the PspGI restriction-modification system in Escherichia coli.

Authors: R Morgan; J p Xiao; S y Xu
Journal: Appl Environ Microbiol Date: 1998-10 Impact factor: 4.792

9. Discovery of a novel restriction endonuclease by genome comparison and application of a wheat-germ-based cell-free translation assay: PabI (5'-GTA/C) from the hyperthermophilic archaeon Pyrococcus abyssi.

Authors: Ken Ishikawa; Miki Watanabe; Toshihiro Kuroita; Ikuo Uchiyama; Janusz M Bujnicki; Bunsei Kawakami; Masaru Tanokura; Ichizo Kobayashi
Journal: Nucleic Acids Res Date: 2005-07-21 Impact factor: 16.971