Literature DB >> 6735746

The element distribution in ultrathin cryosections of cultivated fibroblast cells.

K Zierold, D Schäfer, F Pietruschka.   

Abstract

A preparation method for measurements of the intracellular distribution of elements in tissue culture cells is described which is based on cryofixation, cryoultramicrotomy, cryotransfer and X-ray microanalysis in a scanning transmission electron microscope. Dry weight concentrations of phosphorus, sulfur, chlorine and potassium in the nucleus, the cytoplasm and the mitochondria of L929 fibroblast cells of the mouse are reported. The preparation and quantitation procedures are discussed with respect to present limitations and possible improvements of the method.

Entities:  

Mesh：

Substances：

Year:  1984        PMID： 6735746     DOI： 10.1007/BF00495413

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  19 in total

1.  Potassium transport and content during G1 and S phase following serum stimulation of 3T3 cells.

Authors:  J T Tupper; F Zorgniotti; B Mills
Journal:  J Cell Physiol       Date:  1977-06       Impact factor: 6.384

2.  Standards for quantitative energy dispersive X-ray microanalysis of biological cryosections: validation and application to studies of myocardium.

Authors:  H K Hagler; L E Lopez; J S Flores; R J Lundswick; L M Buja
Journal:  J Microsc       Date:  1983-08       Impact factor: 1.758

3.  Quantification for the x-ray microanalysis of cryosections.

Authors:  T A Hall; B L Gupta
Journal:  J Microsc       Date:  1982-06       Impact factor: 1.758

4.  Changes of intracellular and externally bound cations accompanying serum stimulation of mouse BALB/c 3T3 cells.

Authors:  H Sanui; H Rubin
Journal:  Exp Cell Res       Date:  1982-05       Impact factor: 3.905

5.  Freezing in a propane jet and its application in freeze-fracturing.

Authors:  M Müller; N Meister; H Moor
Journal:  Mikroskopie       Date:  1980-09

Review 6.  X-ray microanalysis of tissue culture cells in SEM and STEM.

Authors:  K Zierold
Journal:  Scan Electron Microsc       Date:  1981

7.  A cooling chain for studies of cryofixed biological specimens by scanning transmission electron microscopy and X-ray microanalysis.

Authors:  K Zierold; R König; K H Olech; D Schäfer; D W Lübbers; K H Müller; H Winter
Journal:  Ultramicroscopy       Date:  1981       Impact factor: 2.689

8.  Electron probe analysis of cultured vascular smooth muscle.

Authors:  M R James-Kracke; B F Sloane; H Shuman; R Karp; A P Somlyo
Journal:  J Cell Physiol       Date:  1980-05       Impact factor: 6.384

Review 9.  The x-ray microanalysis of frozen-hydrated sections in scanning electron microscopy: an evaluation.

Authors:  B L Gupta; T A Hall
Journal:  Tissue Cell       Date:  1981       Impact factor: 2.466

10.  Application of scanning electron microscopy to x-ray analysis of frozen-hydrated sections. II. Analysis of standard solutions and artificial electrolyte gradients.

Authors:  A J Saubermann; R Beeuwkes; P D Peters
Journal:  J Cell Biol       Date:  1981-02       Impact factor: 10.539
View more
  3 in total

1.  Comparative measurements of potassium and chloride with ion-sensitive microelectrodes and x-ray microanalysis in cultured skeletal muscle fibers.

Authors:  H Acker; F Pietruschka; K Zierold
Journal:  In Vitro Cell Dev Biol       Date:  1985-01

2.  Ultrastructural organization of bovine chromaffin cell cortex-analysis by cryofixation and morphometry of aspects pertinent to exocytosis.

Authors:  H Plattner; A R Artalejo; E Neher
Journal:  J Cell Biol       Date:  1997-12-29       Impact factor: 10.539

3.  Iron storage within dopamine neurovesicles revealed by chemical nano-imaging.

Authors:  Richard Ortega; Peter Cloetens; Guillaume Devès; Asunción Carmona; Sylvain Bohic
Journal:  PLoS One       Date:  2007-09-26       Impact factor: 3.240
  3 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.