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Literature DB >> 6735249

Characterisation of a novel proteolytic enzyme localised to goblet cells in rat and man.

E Nexø, S S Poulsen, S N Hansen, P Kirkegaard, P S Olsen.

Abstract

A proteolytic enzyme, ingobsin , purified from rat duodenal extracts is shown to be localised to intestinal goblet cells of both man and rat. Enzyme positive cells decrease in number from duodenum to colon. The enzyme is a 33 000 Mr protein with an isoelectric point of 5.1. The pH optimum for enzymatic activity is 7.4-8.0. Based on substrate specificity for arg-x, lys-x and to a lesser degree tyr-x, on the effect of diisopropylphosphorofluoride , Trasylol and phenylmethylsulfonylfluoride and on proteolytic activity towards intact proteins, ingobsin is classified as a serine proteinase with endoproteolytic activity.

Entities: Chemical Species

Mesh：

Year: 1984 PMID： 6735249 PMCID： PMC1432388 DOI： 10.1136/gut.25.6.656

Source DB: PubMed Journal: Gut ISSN： 0017-5749 Impact factor: 23.059

19 in total

1. High resolution two-dimensional electrophoresis of proteins.

Authors: P H O'Farrell
Journal: J Biol Chem Date: 1975-05-25 Impact factor: 5.157

2. The purification of human enterokinase by affinity chromatography and immunoadsorption. Some observations on its molecular characteristics and comparisons with the pig enzyme.

Authors: D A Grant; J Hermon-Taylor
Journal: Biochem J Date: 1976-05-01 Impact factor: 3.857

3. Effect of proteolytic enzymes on the binding of cobalamin to R protein and intrinsic factor. In vitro evidence that a failure to partially degrade R protein is responsible for cobalamin malabsorption in pancreatic insufficiency.

Authors: R H Allen; B Seetharam; E Podell; D H Alpers
Journal: J Clin Invest Date: 1978-01 Impact factor: 14.808

Characterisation of a novel proteolytic enzyme localised to goblet cells in rat and man.

1. High resolution two-dimensional electrophoresis of proteins.

2. The purification of human enterokinase by affinity chromatography and immunoadsorption. Some observations on its molecular characteristics and comparisons with the pig enzyme.

3. Effect of proteolytic enzymes on the binding of cobalamin to R protein and intrinsic factor. In vitro evidence that a failure to partially degrade R protein is responsible for cobalamin malabsorption in pancreatic insufficiency.

Review 4. Recent studies on the chemistry and biology of epidermal growth factor.

Review 5. Mucins in the human gastrointestinal epithelium: a review.

6. A family of protein-cutting proteins.

7. Comparison of the arginine esteropeptidases associated with the nerve and epidermal growth factor.

8. Characterization of the binding protein for epidermal growth factor.

9. Rat enterokinase: the effect of ions and the localization in the intestine.

10. Subcellular localization of enterokinase in human duodenal mucosa.

1. Localization of a new serine protease, ingobsin, in goblet cells in rat, pig and man.

2. Dipeptidyl peptidase (DPP) IV in rat organs. Comparison of immunohistochemistry and activity histochemistry.