Literature DB >> 6731800

Picogram per cell determination of DNA by flow cytofluorometry.

G M Lee, J T Thornthwaite, E M Rasch.   

Abstract

Using nuclei isolated from less than 0.2 g tissue or 10(7) cells, a method is presented for the quantitative determination of amounts of DNA per cell at the picogram level. This technique is based on the enhanced fluorescence of 4',6-diamidino-2-phenylindole (DAPI) when it binds to DNA. A rapid, one-step nuclear isolation and DNA staining procedure is used to prepare tissue samples for flow cytometric analysis. Frozen tissues give results comparable to those for fresh tissue. Both chicken and trout erythrocyte nuclei were used as reference standards in the determination of amounts of DNA per diploid cell for several mammals and Amazon molly fish. The consistent values obtained for different tissues from the same organism show the accuracy of this method for DNA measurement.

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Year:  1984        PMID： 6731800     DOI： 10.1016/0003-2697(84)90374-9

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  15 in total

1.  Isotropic fractionator: a simple, rapid method for the quantification of total cell and neuron numbers in the brain.

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2.  Maxiprep genomic DNA extractions for molecular epidemiology studies and biorepositories.

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3.  AAV5-mediated gene transfer to the parotid glands of non-human primates.

Authors:  A Voutetakis; C Zheng; A P Cotrim; F Mineshiba; S Afione; N Roescher; W D Swaim; M Metzger; M A Eckhaus; R E Donahue; C E Dunbar; J A Chiorini; B J Baum
Journal:  Gene Ther       Date:  2009-09-17       Impact factor: 5.250

4.  Increase in viral load, viral integration, and gain of telomerase genes during uterine cervical carcinogenesis can be simultaneously assessed by the HPV 16/18 MLPA-assay.

Authors:  Wendy Theelen; Ernst-Jan M Speel; Michael Herfs; Martin Reijans; Guus Simons; Els V Meulemans; Marcella M Baldewijns; Frans C S Ramaekers; Joan Somja; Philippe Delvenne; Anton H N Hopman
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5.  Evidence for DAPI intercalation in CG sites of DNA oligomer [d(CGACGTCG)]2: a 1H NMR study.

Authors:  E Trotta; E D'Ambrosio; G Ravagnan; M Paci
Journal:  Nucleic Acids Res       Date:  1995-04-25       Impact factor: 16.971

6.  Assessing the sensitivity of commercially available fluorophores to the intracellular environment.

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7.  PCR detection of the sheep-associated agent of malignant catarrhal fever.

Authors:  S I Baxter; I Pow; A Bridgen; H W Reid
Journal:  Arch Virol       Date:  1993       Impact factor: 2.574

Review 8.  The search for true numbers of neurons and glial cells in the human brain: A review of 150 years of cell counting.

Authors:  Christopher S von Bartheld; Jami Bahney; Suzana Herculano-Houzel
Journal:  J Comp Neurol       Date:  2016-06-16       Impact factor: 3.215

9.  Recurrence-free survival in breast cancer improved by adjuvant tamoxifen--especially for progesterone receptor positive tumors with a high proliferation.

Authors:  M Fernö; B Baldetorp; P O Bendahl; A Borg; S B Ewers; H Olsson; S Rydén; H Sigurdsson; D Killander
Journal:  Breast Cancer Res Treat       Date:  1995       Impact factor: 4.872

10.  Long-term transduction of miniature pig parotid glands using serotype 2 adeno-associated viral vectors.

Authors:  Bo Hai; Xing Yan; Antonis Voutetakis; Changyu Zheng; Ana P Cotrim; Zhaochen Shan; Gang Ding; Chunmei Zhang; Junji Xu; Corinne M Goldsmith; Sandra Afione; John A Chiorini; Bruce J Baum; Songlin Wang
Journal:  J Gene Med       Date:  2009-06       Impact factor: 4.565
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