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Literature DB >> 6728453

The tubulins of animals, plants, fungi and protists implications for metazoan evolution.

M Little, R F Ludueña, L C Morejohn, C Asnes, E Hoffman.

Abstract

alpha-Tubulin subunits from trout (S. gairdneri) sperm tails, sea urchin (S. purpuratus) cilia, protistan alga (C. elongatum ) flagella and rose (Paul's Scarlet) cytoplasm have been characterized by limited proteolytic cleavage with the enzyme Staphylococcus aureus protease and electrophoresis of the digestion products on SDS-PAGE. The resulting patterns corresponded to either of two major types representative of animal and non-animal alpha-tubulins, respectively. A total of 28 alpha-tubulins have now been characterized by this method. They are classified in this paper according to the type of cleavage pattern generated by the enzyme S. aureus protease. The implications of these results for metazoan evolution are discussed.

Entities: Chemical Species

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Year: 1984 PMID： 6728453

Source DB: PubMed Journal: Orig Life ISSN： 0302-1688

18 in total

4. The colR4 and colR15 beta-tubulin mutations in Chlamydomonas reinhardtii confer altered sensitivities to microtubule inhibitors and herbicides by enhancing microtubule stability.

Authors: M J Schibler; B Huang
Journal: J Cell Biol Date: 1991-05 Impact factor: 10.539

4 in total

The tubulins of animals, plants, fungi and protists implications for metazoan evolution.

1. The preparation and enzymatic hydrolysis of reduced and S-carboxymethylated proteins.

2. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.

3. Rapid visualization of protein bands in preparative SDS-polyacrylamide gels.

4. A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels.

5. Tubulin evolution: two major types of alpha-tubulin.

6. Higher plant tubulin identified by self-assembly into microtubules in vitro.

7. Complete amino acid sequence of beta-tubulin from porcine brain.

8. Nucleotide sequence and evolution of a mammalian alpha-tubulin messenger RNA.

9. Tubulin sequence conservation.

10. Pig sperm tail tubulin. Its extraction and characterization.

Review 1. Microtubule drugs: action, selectivity, and resistance across the kingdoms of life.

2. Characterization of the alpha-tubulin gene family of Arabidopsis thaliana.

3. Identification of multiple tubulins in taxol microtubules purified from carrot suspension cells.

4. The colR4 and colR15 beta-tubulin mutations in Chlamydomonas reinhardtii confer altered sensitivities to microtubule inhibitors and herbicides by enhancing microtubule stability.