Literature DB >> 6722097

Sequential resonance assignments in 1H NMR spectra of oligonucleotides by two-dimensional NMR spectroscopy.

R M Scheek, R Boelens, N Russo, J H van Boom, R Kaptein.   

Abstract

A sequential assignment procedure is outlined, based on two-dimensional NOE ( NOESY ) and two-dimensional J-correlated spectroscopy ( COSY ), for assigning the nonexchangeable proton resonances in NMR spectra of oligonucleotides. As presented here the method is generally applicable to right-handed helical oligonucleotides of intermediate size. We applied it to a lac operator DNA fragment consisting of d( TGAGCGG ) and d( CCGCTCA ) and obtained complete assignments for the adenine H8, guanine H8, cytosine H6 and H5, thymine H6 and 5-methyl, and the deoxyribose H1', H2', H2", H3', and H4' resonances, as well as some H5', H5" (pairwise) assignments. These assignments are required for the analysis of two-dimensional NOE and J-coupling data in terms of the solution structure of oligonucleotides.

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Year:  1984        PMID： 6722097     DOI： 10.1021/bi00302a006

Source DB:  PubMed          Journal:  Biochemistry        ISSN： 0006-2960            Impact factor:   3.162


  42 in total

1.  Structural basis for uracil DNA glycosylase interaction with uracil: NMR study.

Authors:  M Ghosh; N Vinay Kumar; U Varshney; K V Chary
Journal:  Nucleic Acids Res       Date:  2000-05-01       Impact factor: 16.971

2.  Extra thymidine stacks into the d(CTGGTGCGG).d(CCGCCCAG) duplex. An NMR and model-building study.

Authors:  Y T van den Hoogen; A A van Beuzekom; H van den Elst; G A van der Marel; J H van Boom; C Altona
Journal:  Nucleic Acids Res       Date:  1988-04-11       Impact factor: 16.971

3.  1H NMR of 5'CGCGTATATACGCG3', a duplex and a four-membered loop.

Authors:  D A Kallick; D E Wemmer
Journal:  Nucleic Acids Res       Date:  1991-11-11       Impact factor: 16.971

4.  An NMR structural study of deaminated base pairs in DNA.

Authors:  C Carbonnaux; G V Fazakerley; L C Sowers
Journal:  Nucleic Acids Res       Date:  1990-07-25       Impact factor: 16.971

5.  The solution conformations of a mutant trp operator determined by n.m.r. spectroscopy.

Authors:  A N Lane
Journal:  Biochem J       Date:  1991-01-15       Impact factor: 3.857

6.  Alkyl phosphotriester modified oligodeoxyribonucleotides. VI. NMR and UV spectroscopic studies of ethyl phosphotriester (Et) modified Rp-Rp and Sp-Sp duplexes, (d[GGAA(Et)TTCC])2.

Authors:  M F Summers; C Powell; W Egan; R A Byrd; W D Wilson; G Zon
Journal:  Nucleic Acids Res       Date:  1986-09-25       Impact factor: 16.971

7.  Solution structure of oligonucleotides covalently linked to a psoralen derivative.

Authors:  O Bornet; C Prévost; F Vovelle; M Chassignol; N T Thuong; G Lancelot
Journal:  Nucleic Acids Res       Date:  1995-03-11       Impact factor: 16.971

8.  Evidence for DAPI intercalation in CG sites of DNA oligomer [d(CGACGTCG)]2: a 1H NMR study.

Authors:  E Trotta; E D'Ambrosio; G Ravagnan; M Paci
Journal:  Nucleic Acids Res       Date:  1995-04-25       Impact factor: 16.971

9.  Statistical analysis of double NOE transfer pathways in proteins as measured in 3D NOE-NOE spectroscopy.

Authors:  G W Vuister; R Boelens; A Padilla; R Kaptein
Journal:  J Biomol NMR       Date:  1991-11       Impact factor: 2.835

10.  Phosphorothioate-modified oligodeoxyribonucleotides. III. NMR and UV spectroscopic studies of the Rp-Rp, Sp-Sp, and Rp-Sp duplexes, [d(GGSAATTCC)]2, derived from diastereomeric O-ethyl phosphorothioates.

Authors:  L A LaPlanche; T L James; C Powell; W D Wilson; B Uznanski; W J Stec; M F Summers; G Zon
Journal:  Nucleic Acids Res       Date:  1986-11-25       Impact factor: 16.971
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