Literature DB >> 6701520

Bioassay of solubilized Bacillus thuringiensis var. israelensis crystals by attachment to latex beads.

D J Schnell, M A Pfannenstiel, K W Nickerson.   

Abstract

Solubilized crystals of Bacillus thuringiensis var. israelensis were 7000 times less toxic to Aedes aegypti larvae than intact crystals, presumably because mosquito larvae are filter feeders and selectively concentrate particles while excluding water and soluble molecules. A procedure is described whereby soluble toxins are adsorbed to 0.8-micrometer latex beads, with retention of toxicity. The latex bead assay should make it possible to analyze the structure and mode of action of the mosquito toxin.

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Year:  1984        PMID： 6701520     DOI： 10.1126/science.6701520

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  16 in total

1.  Activity of free and clay-bound insecticidal proteins from Bacillus thuringiensis subsp. israelensis against the mosquito Culex pipiens.

Authors:  LanNa Lee; Deepak Saxena; G Stotzky
Journal:  Appl Environ Microbiol       Date:  2003-07       Impact factor: 4.792

2.  The glycoprotein toxin of Bacillus thuringiensis subsp. israelensis indicates a lectinlike receptor in the larval mosquito gut.

Authors:  G Muthukumar; K W Nickerson
Journal:  Appl Environ Microbiol       Date:  1987-11       Impact factor: 4.792

3.  Cadherin, alkaline phosphatase, and aminopeptidase N as receptors of Cry11Ba toxin from Bacillus thuringiensis subsp. jegathesan in Aedes aegypti.

Authors:  Supaporn Likitvivatanavong; Jianwu Chen; Alejandra Bravo; Mario Soberón; Sarjeet S Gill
Journal:  Appl Environ Microbiol       Date:  2010-10-29       Impact factor: 4.792

4.  Structural similarity between the lepidoptera- and diptera-specific insecticidal endotoxin genes of Bacillus thuringiensis subsp. "kurstaki" and "israelensis".

Authors:  L Thorne; F Garduno; T Thompson; D Decker; M Zounes; M Wild; A M Walfield; T J Pollock
Journal:  J Bacteriol       Date:  1986-06       Impact factor: 3.490

5.  Cloning of the gene for the larvicidal toxin of Bacillus sphaericus 2362: evidence for a family of related sequences.

Authors:  P Baumann; L Baumann; R D Bowditch; A H Broadwell
Journal:  J Bacteriol       Date:  1987-09       Impact factor: 3.490

6.  Characterization of mosquitocidal activity of Bacillus thuringiensis subsp. fukuokaensis crystal proteins.

Authors:  Y M Yu; M Ohba; S S Gill
Journal:  Appl Environ Microbiol       Date:  1991-04       Impact factor: 4.792

7.  Immunological relationships among proteins making up the Bacillus thuringiensis subsp. israelensis crystalline toxin.

Authors:  M A Pfannenstiel; G A Couche; E J Ross; K W Nickerson
Journal:  Appl Environ Microbiol       Date:  1986-10       Impact factor: 4.792

8.  Stability of the larvicidal activity of Bacillus thuringiensis subsp. israelensis: amino acid modification and denaturants.

Authors:  M A Pfannenstiel; G A Couche; G Muthukumar; K W Nickerson
Journal:  Appl Environ Microbiol       Date:  1985-11       Impact factor: 4.792

9.  Structural disulfide bonds in the Bacillus thuringiensis subsp. israelensis protein crystal.

Authors:  G A Couche; M A Pfannenstiel; K W Nickerson
Journal:  J Bacteriol       Date:  1987-07       Impact factor: 3.490

10.  Expression in Escherichia coli of a cloned crystal protein gene of Bacillus thuringiensis subsp. israelensis.

Authors:  K M McLean; H R Whiteley
Journal:  J Bacteriol       Date:  1987-03       Impact factor: 3.490
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