Literature DB >> 6700583

Cloning and characterization of small circular DNA from Chinese hamster ovary cells.

S W Stanfield, D R Helinski.   

Abstract

Small polydisperse circular (spc) DNA was isolated and cloned, using BglII from Chinese hamster ovary (CHO) cells. The properties of 47 clones containing at least 43 different BglII fragments are reported. The majority of the clones probably contain entire sequences from individual spcDNA molecules. Most of the clones were homologous to sequences in CHO cell chromosomal DNA, and many were also homologous to mouse LMTK- cell chromosomal sequences. The majority of homologous CHO cell chromosomal sequences were repetitive, although a few may be single copy. Only a small fraction of cloned spcDNA molecules were present in every cell; most occurred less frequently than once in 15 cells. Localization studies indicated that at least a portion of spcDNA is associated with the nucleus in CHO cells.

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Year:  1984        PMID： 6700583      PMCID： PMC368672          DOI： 10.1128/mcb.4.1.173-180.1984

Source DB:  PubMed          Journal:  Mol Cell Biol        ISSN： 0270-7306            Impact factor:   4.272


  25 in total

1.  Isolation and preliminary characterization of the small circular DNA present in African green monkey kidney (BSC-1) cells.

Authors:  R J DeLap; M G Rush; D Zouzias; S Khan
Journal:  Plasmid       Date:  1978-09       Impact factor: 3.466

2.  MOLECULAR SIZE AND CIRCULARITY OF DNA IN CELLS OF MAMMALS AND HIGHER PLANTS.

Authors:  Y HOTTA; A BASSEL
Journal:  Proc Natl Acad Sci U S A       Date:  1965-02       Impact factor: 11.205

3.  Electron microscope analysis of amplifying ribosomal DNA from Xenopus laevis.

Authors:  M Buongiorno-Nardelli; F Amaldi; P A Lava-Sanchez
Journal:  Exp Cell Res       Date:  1976-03-01       Impact factor: 3.905

4.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

5.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

6.  Aspects of the encapsidation of simian virus 40 deoxyribonucleic acid.

Authors:  M Girard; D Stehelin; S Manteuil; J Pages
Journal:  J Virol       Date:  1973-01       Impact factor: 5.103

7.  Simple procedure for isolation of satellite DNA's from tobacco leaves in high yield and demonstration of minicircles.

Authors:  F Y Wong; S G Wildman
Journal:  Biochim Biophys Acta       Date:  1972-01-18

8.  Small circular DNA in Drosophila melanogaster.

Authors:  S Stanfield; D R Helinski
Journal:  Cell       Date:  1976-10       Impact factor: 41.582

9.  Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure.

Authors:  F C Kafatos; C W Jones; A Efstratiadis
Journal:  Nucleic Acids Res       Date:  1979-11-24       Impact factor: 16.971

10.  Change in quantity and size distribution of small circular DNAs during development of chicken bursa.

Authors:  R J DeLap; M G Rush
Journal:  Proc Natl Acad Sci U S A       Date:  1978-12       Impact factor: 11.205
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  21 in total

1.  Hamster line and ALU-equivalent sequences are present in the small polydispersed circular DNA population of CHO cells.

Authors:  C Miles; M Meuth
Journal:  Nucleic Acids Res       Date:  1989-07-25       Impact factor: 16.971

2.  Characterization of cloned human alphoid satellite with an unusual monomeric construction: evidence for enrichment in HeLa small polydisperse circular DNA.

Authors:  R S Jones; S S Potter
Journal:  Nucleic Acids Res       Date:  1985-02-11       Impact factor: 16.971

3.  Transposon-like sequences in extrachromosomal circular DNA from mouse thymocytes.

Authors:  S Fujimoto; T Tsuda; M Toda; H Yamagishi
Journal:  Proc Natl Acad Sci U S A       Date:  1985-04       Impact factor: 11.205

4.  Discrete size classes of monkey extrachromosomal circular DNA containing the L1 family of long interspersed nucleotide sequences are produced by a general non-sequence specific mechanism.

Authors:  C W Schindler; M G Rush
Journal:  Nucleic Acids Res       Date:  1985-11-25       Impact factor: 16.971

5.  Multiple mechanisms generate extrachromosomal circular DNA in Chinese hamster ovary cells.

Authors:  S W Stanfield; D R Helinski
Journal:  Nucleic Acids Res       Date:  1986-04-25       Impact factor: 16.971

6.  DNA sequence homology between the terminal inverted repeats of Shope fibroma virus and an endogenous cellular plasmid species.

Authors:  C Upton; G McFadden
Journal:  Mol Cell Biol       Date:  1986-01       Impact factor: 4.272

7.  G-repeats: a novel hamster sine family.

Authors:  C Miles; M Meuth
Journal:  Nucleic Acids Res       Date:  1989-09-25       Impact factor: 16.971

8.  Formation of an inverted duplication can be an initial step in gene amplification.

Authors:  J C Ruiz; G M Wahl
Journal:  Mol Cell Biol       Date:  1988-10       Impact factor: 4.272

9.  Molecular cloning and characterization of small polydisperse circular DNA from mouse 3T6 cells.

Authors:  P Sunnerhagen; R M Sjöberg; A L Karlsson; L Lundh; G Bjursell
Journal:  Nucleic Acids Res       Date:  1986-10-24       Impact factor: 16.971

10.  Characterization of repetitive sequence families in mouse heart small polydisperse circular DNAs: age-related studies.

Authors:  S C Flores; P Sunnerhagen; T K Moore; J W Gaubatz
Journal:  Nucleic Acids Res       Date:  1988-05-11       Impact factor: 16.971
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