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Literature DB >> 670

phiX174 DNA-dependent DNA synthesis in vitro: requirement for P1 ban protein in dnaB mutant extracts of Escherichia coli.

H Schuster, M Mikolajczyk, J Rohrschneider, B Geschke.

Abstract

Ammonium sulfate fractionation of crude extracts of E. coli yields a soluble enzyme fraction (about 25-fold purification) that catalyzes the conversion of phiX174 single-stranded DNA to duplex DNA. The reaction is rifampicin-resistant, requires single-stranded DNA, Mg++, deoxynucleoside triphosphates, and ATP, and is stimulated by KCl. Such soluble enzyme fractions were prepared from E. coli strains carrying the prophage mutant P1bac, in which the viral dnaB analog (ban) protein is expressed constitutively, or P1bacban, in which the expression of ban protein is prevented. DNA-synthesizing activity of ban protein containing fractions from wild-type or dnaB(P1bac) lysogens was more temperature-resistant than that from E. coli containing only wild-type dnaB protein, whereas that from dnaB(P1bacban) lysogens of dnaB cells was extremely thermolabile. It is suggested that the temperature-resistant DNA synthesis with fractions from P1bac lysogens is mediated by the P1 ban protein.

Entities: Chemical Species

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Year: 1975 PMID： 670 PMCID： PMC433105 DOI： 10.1073/pnas.72.10.3907

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

15 in total

1. Analysis of dnaB function of Escherichia coli K12 and the dnaB-like function of P1 prophage.

Authors: T Ogawa
Journal: J Mol Biol Date: 1975-05-25 Impact factor: 5.469

2. A dnaB analog specified by bacteriophage P1.

Authors: R D'Ari; A Jaffé-Brachet; D Touati-Schwartz; M B Yarmolinsky
Journal: J Mol Biol Date: 1975-05-25 Impact factor: 5.469

3. Isolation and characterization of thermosensitive Escherichia coli mutants defective in deoxyribonucleic acid replication.

Authors: J A Wechsler; V Nüsslein; B Otto; A Klein; F Bonhoeffer; R Herrmann; L Gloger; H Schaller
Journal: J Bacteriol Date: 1973-03 Impact factor: 3.490

4. Isolation of a complementing activity for a dna-B mutant.

Authors: M Koiyama; E Milewski
Journal: Biochem Biophys Res Commun Date: 1973-08-06 Impact factor: 3.575

5. DNA synthesis in P1 infected E. coli mutants temperature-sensitive in DNA replication.

Authors: D Beyersmann; H Schuster
Journal: Mol Gen Genet Date: 1972

Review 6. Multienzyme systems of DNA replication.

Authors: R Schekman; A Weiner; A Kornberg
Journal: Science Date: 1974-12-13 Impact factor: 47.728

7. RNA synthesis initiates in vitro conversion of M13 DNA to its replicative form.

Authors: W Wickner; D Brutlag; R Schekman; A Kornberg
Journal: Proc Natl Acad Sci U S A Date: 1972-04 Impact factor: 11.205

8. DRUG RESISTANCE OF ENTERIC BACTERIA. IV. ACTIVE TRANSDUCING BACTERIOPHAGE P1 CM PRODUCED BY THE COMBINATION OF R FACTOR WITH BACTERIOPHAGE P1.

Authors: E KONDO; S MITSUHASHI
Journal: J Bacteriol Date: 1964-11 Impact factor: 3.490

7. Replication of the colicin E1 plasmid in extracts of Escherichia coli: uncoupling of leading strand from lagging strand synthesis.

Authors: W L Staudenbauer; E Scherzinger; E Lanka
Journal: Mol Gen Genet Date: 1979

phiX174 DNA-dependent DNA synthesis in vitro: requirement for P1 ban protein in dnaB mutant extracts of Escherichia coli.

1. Analysis of dnaB function of Escherichia coli K12 and the dnaB-like function of P1 prophage.

2. A dnaB analog specified by bacteriophage P1.

3. Isolation and characterization of thermosensitive Escherichia coli mutants defective in deoxyribonucleic acid replication.

4. Isolation of a complementing activity for a dna-B mutant.

5. DNA synthesis in P1 infected E. coli mutants temperature-sensitive in DNA replication.

Review 6. Multienzyme systems of DNA replication.

7. RNA synthesis initiates in vitro conversion of M13 DNA to its replicative form.

8. DRUG RESISTANCE OF ENTERIC BACTERIA. IV. ACTIVE TRANSDUCING BACTERIOPHAGE P1 CM PRODUCED BY THE COMBINATION OF R FACTOR WITH BACTERIOPHAGE P1.

9. Studies on in vitro DNA synthesis. Isolation of DNA B gene product from Escherichia coli.

10. Conversion of phiX174 viral DNA to double-stranded form by purified Escherichia coli proteins.

1. Analogs of the dnaB gene of Escherichia coli K-12 associated with conjugative R plasmids.

2. Escherichia coli dnaB mutant defective in DNA initiation: isolation and properties of the dnaB protein.

3. DNA synthesis in an Escherichia coli dna B dnaC mutant.

4. Cloning, expression, and characterization of the icd gene in the immI operon of bacteriophage P1.

5. Phage P4 DNA replication in vitro.

6. Discriminatory function of ribonuclease H in the selective initiation of plasmid DNA replication.

7. Replication of the colicin E1 plasmid in extracts of Escherichia coli: uncoupling of leading strand from lagging strand synthesis.

8. Replication of the broad host range plasmid RSF1010: requirement for three plasmid-encoded proteins.

9. The dnaC protein of Escherichia coli. Purification, physical properties and interaction with dnaB protein.

10. Replication of small plasmids in extracts of Escherichia coli.