| Literature DB >> 4274130 |
M Wright, S Wickner, J Hurwitz.
Abstract
Formation of duplex DNA from varphiX174 single-stranded DNA by extracts of E. coli was previously shown to require the gene product of dna B. Using as an assay the stimulation of varphiX174 DNA-dependent synthesis in inactivated extracts of dna B temperature-sensitive cells, we purified the dna B gene product from wild-type E. coli as well as from a dna B temperature-sensitive mutant. The dna B temperature-sensitive gene product is more thermolabile than its wild-type counterpart.Entities:
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Year: 1973 PMID: 4274130 PMCID: PMC427183 DOI: 10.1073/pnas.70.11.3120
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN: 0027-8424 Impact factor: 11.205