Literature DB >> 669734

Characterization of normal human embryo cells grown to over 100 population doublings.

J A Poiley, R F Schuman, R J Pienta.   

Abstract

Normal human embryonic cells were subcultured for over 100 population doublings without modification of the basic medium. The cells were evaluated for growth rate, confluent density, chromosome stability, growth in soft agar, ability to hydrolyze casein and tumorigenicity. The cells possessed the characteristics of normal cells. The batch of serum used to supplement the medium was found to be of primary importance in the long-term growth of this cell culture.

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Year:  1978        PMID： 669734     DOI： 10.1007/BF02616101

Source DB:  PubMed          Journal:  In Vitro        ISSN： 0073-5655


  30 in total

1.  In vitro cultivation of cells from aneuploid human embryos. Initiation of cell lines and longevity of the cultures.

Authors:  A Boué; J Boué; S Cure; C Deluchat; N Perraudin
Journal:  In Vitro       Date:  1975 Nov-Dec

2.  Fetal bovine serum: a multivariate standard.

Authors:  K V Honn; J A Singley; W Chavin
Journal:  Proc Soc Exp Biol Med       Date:  1975-06

3.  The influence of culture medium volume on cell density and lifespan of human diploid fibroblasts.

Authors:  J M Ryan; B B Sharf; V J Cristofalo
Journal:  Exp Cell Res       Date:  1975-03-15       Impact factor: 3.905

4.  Insulin and hydrocortisone effects on viability and glycogen stores of postnatal rat liver organ culture.

Authors:  G B Reed; J W Grisham
Journal:  Lab Invest       Date:  1975-09       Impact factor: 5.662

5.  SERUM ALBUMIN SUPPLEMENTED MEDIUM FOR LONG TERM CULTIVATION OF MAMMALIAN FIBROBLAST STRAINS.

Authors:  G J TODARO; H GREEN
Journal:  Proc Soc Exp Biol Med       Date:  1964-07

6.  Aging of human fibroblasts in vitro. Correlations between DNA synthetic ability and cell size.

Authors:  P D Bowman; R L Meek; C W Daniel
Journal:  Exp Cell Res       Date:  1975-06       Impact factor: 3.905

7.  In vitro correlates of transformation in C3H/10T1/2 clone 8 mouse cells.

Authors:  P A Jones; W E Laug; A Gardner; C A Nye; L M Fink; W F Benedict
Journal:  Cancer Res       Date:  1976-08       Impact factor: 12.701

8.  Lack of correlation between fibrinolysis and the transformed state of cultured mammalian cells.

Authors:  D M Mott; P H Fabisch; B P Sani; S Sorof
Journal:  Biochem Biophys Res Commun       Date:  1974-11-27       Impact factor: 3.575

9.  Fibrinolytic activity in a human fibrosarcoma cell line and evidence for the induction of plasminogen activator secretion during tumor formation.

Authors:  P A Jones; W E Laug; W F Benedict
Journal:  Cell       Date:  1975-10       Impact factor: 41.582

10.  Insulin effect on the cell cycle: analysis of the kinetics of growth parameters in confluent chick cells.

Authors:  M H Teng; J C Bartholomew; M J Bissell
Journal:  Proc Natl Acad Sci U S A       Date:  1976-09       Impact factor: 11.205
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  1 in total

1.  Effect of fetal bovine serum on 3-methylcholanthrene-induced transformation of hamster cells in vitro.

Authors:  R F Schuman; R J Pienta; J A Poiley; W B Lebherz
Journal:  In Vitro       Date:  1979-09
  1 in total

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