Literature DB >> 535917

Effect of fetal bovine serum on 3-methylcholanthrene-induced transformation of hamster cells in vitro.

R F Schuman, R J Pienta, J A Poiley, W B Lebherz.   

Abstract

Eighteen lots of fetal bovine serum were tested for their ability to support clonal growth and 3-methylcholanthrene-induced morphological transformation of hamster embryo cells in vitro. Most of them supported cloning efficiencies of over 11%. However, cloning efficiency alone was an inadequate criterion for selecting serum for transformation studies, since no transformation was observed with some lots, even though their cloning efficiencies were over 16%. This shows the importance of pretesting serum for its ability to support morphological transformation before it is used in mammalian cell carcinogenesis tests.

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Year:  1979        PMID： 535917     DOI： 10.1007/BF02618253

Source DB:  PubMed          Journal:  In Vitro        ISSN： 0073-5655


  12 in total

1.  Fetal bovine serum: a multivariate standard.

Authors:  K V Honn; J A Singley; W Chavin
Journal:  Proc Soc Exp Biol Med       Date:  1975-06

2.  Relationship between somatic mutation and neoplastic transformation.

Authors:  J C Barrett; P O Ts'o
Journal:  Proc Natl Acad Sci U S A       Date:  1978-07       Impact factor: 11.205

3.  Plating efficiency for primary hamster embryo cells as an index of efficacy of fetal bovne serum for cell culture.

Authors:  C R Goodheart; B C Castro; A Giviers; P R Regnier
Journal:  Appl Microbiol       Date:  1973-10

4.  Quality control studies on fetal bovine serum used in tissue culture.

Authors:  C W Boone; N Mantel; T D Caruso; E Kazam; R E Stevenson
Journal:  In Vitro       Date:  1971 Nov-Dec

5.  Quantitative studies of in vitro transformation by chemical carcinogens.

Authors:  J A DiPaolo; P Donovan; R Nelson
Journal:  J Natl Cancer Inst       Date:  1969-05       Impact factor: 13.506

6.  A quantitative system for assay of malignant transformation by chemical carcinogens using a clone derived from BALB-3T3.

Authors:  T Kakunaga
Journal:  Int J Cancer       Date:  1973-09-15       Impact factor: 7.396

7.  Transformation of cell cultures as an indication of the carcinogenic potential of chemicals.

Authors:  A E Freeman; E K Weisburger; J H Weisburger; R G Wolford; J M Maryak; R J Huebner
Journal:  J Natl Cancer Inst       Date:  1973-09       Impact factor: 13.506

8.  Characterization of normal human embryo cells grown to over 100 population doublings.

Authors:  J A Poiley; R F Schuman; R J Pienta
Journal:  In Vitro       Date:  1978-05

9.  Morphological transformation of early passage golden Syrian hamster embryo cells derived from cryopreserved primary cultures as a reliable in vitro bioassay for identifying diverse carcinogens.

Authors:  R J Pienta; J A Poiley; W B Lebherz
Journal:  Int J Cancer       Date:  1977-05-15       Impact factor: 7.396

10.  Effects of steroid hormones in fetal bovine serum on plating ang cloning of human cells in vitro.

Authors:  G E Milo; W B Malarkey; J E Powell; J R Blakeslee; D S Yohn
Journal:  In Vitro       Date:  1976-01
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  1 in total

1.  Establishment and characterization of a new cell line from a hereditary renal rat tumor.

Authors:  P Eker; R Eker; J V Johannessen
Journal:  In Vitro       Date:  1983-06
  1 in total

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