Literature DB >> 6693575

Detection of Clostridium difficile in faeces by direct gas liquid chromatography.

P N Levett.   

Abstract

Stool specimens examined for the presence of Clostridium difficile and its cytotoxin were screened by gas liquid chromatography for the presence of volatile fatty acids and p-cresol. Twenty seven of 110 (25%) stools yielded C difficile or cytotoxin; iso-valeric acid was detected in 63/110 (57%) and iso-caproic acid in 18/110 (16%) stools. Para-cresol was found in 24/71 (34%) stools examined. Iso-valeric acid was detected in 85% of stools positive for C difficile, whereas iso-caproic acid (41%) and p-cresol (52%) were found in much lower numbers of C difficile-positive stools. It is concluded that gas chromatographic detection of volatile fatty acids or p-cresol in faeces are not satisfactory screening tests for the presence of C difficile.

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Year:  1984        PMID： 6693575      PMCID： PMC498663          DOI： 10.1136/jcp.37.2.117

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN： 0021-9746            Impact factor:   3.411


  15 in total

1.  Gas-liquid chromatography as screening test for Clostridium difficile.

Authors:  C Potvliege; M Labbé; E Yourassowsky
Journal:  Lancet       Date:  1981-11-14       Impact factor: 79.321

2.  Rapid detection of Clostridium difficile toxin in human feces.

Authors:  R W Ryan; I Kwasnik; R C Tilton
Journal:  J Clin Microbiol       Date:  1980-12       Impact factor: 5.948

3.  Simplified procedure for the routine isolation of Clostridium difficile from faeces.

Authors:  S P Borriello; P Honour
Journal:  J Clin Pathol       Date:  1981-10       Impact factor: 3.411

4.  Detection of Clostridium difficile toxin by counterimmunoelectrophoresis: a note of caution.

Authors:  I R Poxton; M D Byrne
Journal:  J Clin Microbiol       Date:  1981-09       Impact factor: 5.948

5.  The end products of the metabolism of aromatic amino acids by Clostridia.

Authors:  S R Elsden; M G Hilton; J M Waller
Journal:  Arch Microbiol       Date:  1976-04-01       Impact factor: 2.552

6.  Prospective study of gram-stained stool smears in diagnosis of Clostridium difficile colitis.

Authors:  C J Shanholtzer; L R Peterson; M N Olson; D N Gerding
Journal:  J Clin Microbiol       Date:  1983-05       Impact factor: 5.948

7.  Selective and differential medium for isolation of Clostridium difficile.

Authors:  W L George; V L Sutter; D Citron; S M Finegold
Journal:  J Clin Microbiol       Date:  1979-02       Impact factor: 5.948

8.  Cultures for Clostridium difficile in stools containing a cytotoxin neutralized by Clostridium sordellii antitoxin.

Authors:  S H Willey; J G Bartlett
Journal:  J Clin Microbiol       Date:  1979-12       Impact factor: 5.948

9.  Rapid detection and presumptive identification of Clostridium difficile by p-cresol production on a selective medium.

Authors:  K D Phillips; P A Rogers
Journal:  J Clin Pathol       Date:  1981-06       Impact factor: 3.411

10.  Identification of toxigenic Clostridium difficile by counterimmunoelectrophoresis.

Authors:  D F Welch; S K Menge; J M Matsen
Journal:  J Clin Microbiol       Date:  1980-05       Impact factor: 5.948
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  9 in total

1.  Effect of antibiotic concentration in a selective medium on the isolation of Clostridium difficile from faecal specimens.

Authors:  P N Levett
Journal:  J Clin Pathol       Date:  1985-02       Impact factor: 3.411

2.  Incidence and significance of Clostridium difficile in hospitalized cancer patients.

Authors:  M Gérard; N Defresne; D Daneau; P Van der Auwera; M Delmée; A M Bourguignon; F Meunier
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1988-04       Impact factor: 3.267

Review 3.  Clostridium difficile: its disease and toxins.

Authors:  D M Lyerly; H C Krivan; T D Wilkins
Journal:  Clin Microbiol Rev       Date:  1988-01       Impact factor: 26.132

4.  Presumptive identification of Clostridium difficile by detection of p-cresol in prepared peptone yeast glucose broth supplemented with p-hydroxyphenylacetic acid.

Authors:  G Sivsammye; H V Sims
Journal:  J Clin Microbiol       Date:  1990-08       Impact factor: 5.948

5.  Evaluation of gas-liquid chromatography for the rapid diagnosis of Clostridium difficile associated disease.

Authors:  P Gianfrilli; A Pantosti; I Luzzi
Journal:  J Clin Pathol       Date:  1985-06       Impact factor: 3.411

Review 6.  Clostridium difficile: clinical disease and diagnosis.

Authors:  F C Knoop; M Owens; I C Crocker
Journal:  Clin Microbiol Rev       Date:  1993-07       Impact factor: 26.132

7.  Enzyme immunoassay for detection of Clostridium difficile toxins A and B in patients with antibiotic-associated diarrhoea and colitis.

Authors:  B Aronsson; M Granström; R Möllby; C E Nord
Journal:  Eur J Clin Microbiol       Date:  1985-04       Impact factor: 3.267

Review 8.  Laboratory diagnosis of Clostridium difficile-associated diarrhoea.

Authors:  R A Bowman; T V Riley
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1988-08       Impact factor: 3.267

9.  Identification of Clostridium difficile in stool specimens by culture-enhanced gas-liquid chromatography.

Authors:  L L Johnson; L V McFarland; P Dearing; V Raisys; F D Schoenknecht
Journal:  J Clin Microbiol       Date:  1989-10       Impact factor: 5.948
  9 in total

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