Literature DB >> 6689067

Isolation and characterisation of cDNA clones for the A alpha- and gamma-chains of human fibrinogen.

A M Imam, M A Eaton, R Williamson, S Humphries.   

Abstract

cDNA clones coding for the A alpha- and gamma-chains of human fibrinogen have been isolated from an adult liver cDNA library. Clones were identified by hybridisation with mixtures of synthetic oligonucleotides 17 bases long, predicted using amino acid sequence data for each chain. The cDNA insert sizes are 1,950bp for A alpha-fibrinogen and 950bp for gamma-fibrinogen. The clones do not show any cross-hybridisation. Each cDNA hybridises to a unique sequence in the human genome. In adult human liver, Northern blots give an estimated messenger RNA size of 2.6kb for A alpha-fibrinogen and 1.8kb for gamma-fibrinogen.

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Year:  1983        PMID： 6689067      PMCID： PMC326493          DOI： 10.1093/nar/11.21.7427

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  22 in total

1.  Gel electrophoresis of restriction fragments.

Authors:  E Southern
Journal:  Methods Enzymol       Date:  1979       Impact factor: 1.600

2.  Amino acid sequence of human fibrin. Preliminary note on the completion of the gamma-chain sequence.

Authors:  F Lottspeich; A Henschen
Journal:  Hoppe Seylers Z Physiol Chem       Date:  1977-07

3.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

4.  Disulfide bridges in nh2 -terminal part of human fibrinogen.

Authors:  B Blombäck; B Hessel; D Hogg
Journal:  Thromb Res       Date:  1976-05       Impact factor: 3.944

5.  A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Authors:  H C Birnboim; J Doly
Journal:  Nucleic Acids Res       Date:  1979-11-24       Impact factor: 16.971

Review 6.  Population-based distributions of haemostatic variables.

Authors:  T W Meade; W R North; R Chakrabarti; A P Haines; Y Stirling
Journal:  Br Med Bull       Date:  1977-09       Impact factor: 4.291

7.  The amino acid sequence of the alpha-chain of human fibrinogen.

Authors:  R F Doolittle; K W Watt; B A Cottrell; D D Strong; M Riley
Journal:  Nature       Date:  1979-08-09       Impact factor: 49.962

8.  Amino acid sequence of the beta chain of human fibrinogen.

Authors:  K W Watt; T Takagi; R F Doolittle
Journal:  Biochemistry       Date:  1979-01-09       Impact factor: 3.162

9.  A physical map of the DNA regions flanking the rabbit beta-globin gene.

Authors:  A J Jeffreys; R A Flavell
Journal:  Cell       Date:  1977-10       Impact factor: 41.582

10.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205
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  5 in total

1.  Inverted duplication of JH associated with chromosome 14 translocation and T-cell leukemia in ataxia-telangiectasia.

Authors:  J P Johnson; R A Gatti; T S Sears; R L White
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2.  The association of combined alpha and beta fibrinogen genotype on plasma fibrinogen levels in smokers and non-smokers.

Authors:  A E Thomas; F R Green; H Lamlum; S E Humphries
Journal:  J Med Genet       Date:  1995-08       Impact factor: 6.318

3.  The positions of three restriction fragment length polymorphisms on chromosome 4 relative to known genetic markers.

Authors:  T C Gilliam; P Scambler; T Robbins; C Ingle; R Williamson; K E Davies
Journal:  Hum Genet       Date:  1984       Impact factor: 4.132

4.  The identification of a DNA polymorphism of the alpha fibrinogen gene, and the regional assignment of the human fibrinogen genes to 4q26-qter.

Authors:  S E Humphries; A M Imam; T P Robbins; M Cook; B Carritt; C Ingle; R Williamson
Journal:  Hum Genet       Date:  1984       Impact factor: 4.132

5.  Linkage studies between polymorphic markers on chromosome 4 and cystic fibrosis.

Authors:  P Scambler; T Robbins; C Gilliam; A Boylston; P Tippett; R Williamson; K E Davies
Journal:  Hum Genet       Date:  1985       Impact factor: 4.132
  5 in total

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