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Literature DB >> 6672022

Analysis of phospholipids in human semen by high-performance liquid chromatography.

Abstract

A sensitive method for the separation of phospholipids by a high-performance liquid chromatographic procedure is described. The chromatographic separation was achieved on a 25-cm column packed with Bio-Sil HP-10 coupled with a pre-column packed with Si-100 Polyol. Phosphatidylinositol, phosphatidylglycerol, cardiolipin, phosphatidylserine, phosphatidylethanolamine, phosphatidylcholine, sphingomyelin, lysophosphatidylethanolamine and lysophosphatidylcholine were completely separated and quantitated. The eluted phospholipids were monitored at 203 nm. The method was shown to be applicable to the analysis of phospholipids from human semen.

Entities: Chemical Gene Species

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Year: 1983 PMID： 6672022 DOI： 10.1016/s0378-4347(00)85062-8

Source DB: PubMed Journal: J Chromatogr

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Cited

13 in total

1. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis of cardiolipin extracted from detergent-solubilized mitochondrial electron transfer complexes.

Authors: Tiffany McDonald-Marsh; Christopher A Carroll; Neal C Robinson; Andrej Musatov
Journal: Anal Biochem Date: 2006-09-22 Impact factor: 3.365

2. Separation and quantification of heart and liver phospholipid classes by high-performance liquid chromatography using a new light-scattering detector.

Authors: P Juanéda; G Rocquelin; P O Astorg
Journal: Lipids Date: 1990-11 Impact factor: 1.880

3. Testicular and epididymal toxicity induced by benzo(a)pyrene, alcohol, and their combination in Wistar rats.

Authors: K Pratap Reddy; P Sreenivasula Reddy
Journal: Toxicol Res (Camb) Date: 2015-11-25 Impact factor: 3.524

4. Differential effect of N-ethyl maleimide on delta6-desaturase activity in human fetal liver toward fatty acids of the n-6 and n-3 series.

Authors: A Rodriguez; P Sarda; P Boulot; C L Leger; B Descomps
Journal: Lipids Date: 1999-01 Impact factor: 1.880

9. Different respiratory-defective phenotypes of Neurospora crassa and Saccharomyces cerevisiae after inactivation of the gene encoding the mitochondrial acyl carrier protein.

Authors: R Schneider; M Massow; T Lisowsky; H Weiss
Journal: Curr Genet Date: 1995-12 Impact factor: 3.886

10. Complete separation of phospholipids from human heart combining two HPLC methods.

Authors: P Juanéda; G Rocquelin
Journal: Lipids Date: 1986-03 Impact factor: 1.880

Analysis of phospholipids in human semen by high-performance liquid chromatography.

1. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis of cardiolipin extracted from detergent-solubilized mitochondrial electron transfer complexes.

2. Separation and quantification of heart and liver phospholipid classes by high-performance liquid chromatography using a new light-scattering detector.

3. Testicular and epididymal toxicity induced by benzo(a)pyrene, alcohol, and their combination in Wistar rats.

4. Differential effect of N-ethyl maleimide on delta6-desaturase activity in human fetal liver toward fatty acids of the n-6 and n-3 series.

5. Cytoplasmic lipid bodies of human neutrophilic leukocytes.

6. Glutathione peroxidase 4 differentially regulates the release of apoptogenic proteins from mitochondria.

7. Removal of bound Triton X-100 from purified bovine heart cytochrome bc1.

8. Evidence for diplasmalogen as the major component of rabbit sperm phosphatidylethanolamine.

9. Different respiratory-defective phenotypes of Neurospora crassa and Saccharomyces cerevisiae after inactivation of the gene encoding the mitochondrial acyl carrier protein.

10. Complete separation of phospholipids from human heart combining two HPLC methods.