Literature DB >> 6671475

Purification and crystallization of mammalian lens gamma-crystallins.

C Slingsby, L R Miller.   

Abstract

Monomeric crystallins, beta s and several members of the gamma-crystallin family, occur in different relative proportions in a way which is related to the age of the lens cells. Methods of purification of large amounts of these different low molecular weight protein components from young and old bovine lenses are described along with details of crystallization of several gamma-fractions. Purification procedures have been developed for rabbit gamma-crystallins. The chromatographic methods achieve separation of several bovine and rabbit gamma-crystallins which have very similar electrophoretic mobilities. However, on storage, many electrophoretic variants are generated from some of these fractions.

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Year:  1983        PMID： 6671475     DOI： 10.1016/0014-4835(83)90028-3

Source DB:  PubMed          Journal:  Exp Eye Res        ISSN： 0014-4835            Impact factor:   3.467


  9 in total

Review 1.  Protein interactions in the calf eye lens: interactions between beta-crystallins are repulsive whereas in gamma-crystallins they are attractive.

Authors:  A Tardieu; F Vérétout; B Krop; C Slingsby
Journal:  Eur Biophys J       Date:  1992       Impact factor: 1.733

Review 2.  A superfamily in the mammalian eye lens: the beta/gamma-crystallins.

Authors:  G L van Rens; W W de Jong; H Bloemendal
Journal:  Mol Biol Rep       Date:  1992-02       Impact factor: 2.316

3.  Binary-liquid phase separation of lens protein solutions.

Authors:  M L Broide; C R Berland; J Pande; O O Ogun; G B Benedek
Journal:  Proc Natl Acad Sci U S A       Date:  1991-07-01       Impact factor: 11.205

4.  Near-infrared Fourier transform Raman and conventional Raman studies of calf gamma-crystallins in the lyophilized state and in solution.

Authors:  W L Chen; S M Nie; J F Kuck; N T Yu
Journal:  Biophys J       Date:  1991-08       Impact factor: 4.033

5.  Suppression of phase separation in solutions of bovine gamma IV-crystallin by polar modification of the sulfur-containing amino acids.

Authors:  J Pande; C Berland; M Broide; O Ogun; J Melhuish; G Benedek
Journal:  Proc Natl Acad Sci U S A       Date:  1991-06-01       Impact factor: 11.205

6.  Opacification of gamma-crystallin solutions from calf lens in relation to cold cataract formation.

Authors:  R J Siezen; M R Fisch; C Slingsby; G B Benedek
Journal:  Proc Natl Acad Sci U S A       Date:  1985-03       Impact factor: 11.205

7.  A locus for a human hereditary cataract is closely linked to the gamma-crystallin gene family.

Authors:  N H Lubsen; J H Renwick; L C Tsui; M L Breitman; J G Schoenmakers
Journal:  Proc Natl Acad Sci U S A       Date:  1987-01       Impact factor: 11.205

8.  The reaction of glutathione with the eye-lens protein gamma-crystallin.

Authors:  C Slingsby; L Miller
Journal:  Biochem J       Date:  1985-08-15       Impact factor: 3.857

9.  Human lens gamma-crystallins: isolation, identification, and characterization of the expressed gene products.

Authors:  R J Siezen; J A Thomson; E D Kaplan; G B Benedek
Journal:  Proc Natl Acad Sci U S A       Date:  1987-09       Impact factor: 11.205
  9 in total

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