Literature DB >> 6670747

A method for the ligation of DNA following isolation from low melting temperature agarose.

D M Burns, I R Beacham.   

Abstract

A rapid, simple, and reliable method is presented for the isolation and subsequent ligation of DNA from agarose gels. The technique involves the use of low melting temperature agarose, but with the inclusion of bovine serum albumin or gelatin to the ligation reaction.

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Year:  1983        PMID： 6670747     DOI： 10.1016/0003-2697(83)90728-5

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  17 in total

1.  STRU-cloning: a fast, inexpensive and efficient cloning procedure applicable to both small scale and structural genomics size cloning.

Authors:  Dom Bellini; Anthony P Fordham-Skelton; Miroslav Z Papiz
Journal:  Mol Biotechnol       Date:  2011-05       Impact factor: 2.695

2.  Rapid and efficient subcloning of DNA without dephosphorylation or gel electrophoresis.

Authors:  Qilong Xu; Deqing Zhang; Bruce Downie
Journal:  Mol Biotechnol       Date:  2005-02       Impact factor: 2.695

3.  Analysis of the Escherichia coli gene encoding L-asparaginase II, ansB, and its regulation by cyclic AMP receptor and FNR proteins.

Authors:  M P Jennings; I R Beacham
Journal:  J Bacteriol       Date:  1990-03       Impact factor: 3.490

4.  Fine analysis of the chromatin structure of the yeast SUC2 gene and of its changes upon derepression. Comparison between the chromosomal and plasmid-inserted genes.

Authors:  J E Pérez-Ortín; F Estruch; E Matallana; L Franco
Journal:  Nucleic Acids Res       Date:  1987-09-11       Impact factor: 16.971

5.  Molecular cloning and amino acid sequence of the precursor form of bovine adrenodoxin: evidence for a previously unidentified COOH-terminal peptide.

Authors:  T Okamura; M E John; M X Zuber; E R Simpson; M R Waterman
Journal:  Proc Natl Acad Sci U S A       Date:  1985-09       Impact factor: 11.205

6.  Isolation and sequence analysis of the gene (cpdB) encoding periplasmic 2',3'-cyclic phosphodiesterase.

Authors:  J Liu; D M Burns; I R Beacham
Journal:  J Bacteriol       Date:  1986-03       Impact factor: 3.490

7.  Nucleotide sequence and transcriptional analysis of the E. coli ushA gene, encoding periplasmic UDP-sugar hydrolase (5'-nucleotidase): regulation of the ushA gene, and the signal sequence of its encoded protein product.

Authors:  D M Burns; I R Beacham
Journal:  Nucleic Acids Res       Date:  1986-05-27       Impact factor: 16.971

8.  Cloning of the citrate permease gene of Lactococcus lactis subsp. lactis biovar diacetylactis and expression in Escherichia coli.

Authors:  F Sesma; D Gardiol; A P de Ruiz Holgado; D de Mendoza
Journal:  Appl Environ Microbiol       Date:  1990-07       Impact factor: 4.792

9.  DNase I sensitivity of the chromatin of the yeast SUC2 gene for invertase.

Authors:  J E Pérez-Ortin; F Estruch; E Matallana; L Franco
Journal:  Mol Gen Genet       Date:  1986-12

10.  Resistance to chloramphenicol in Proteus mirabilis by expression of a chromosomal gene for chloramphenicol acetyltransferase.

Authors:  I G Charles; S Harford; J F Brookfield; W V Shaw
Journal:  J Bacteriol       Date:  1985-10       Impact factor: 3.490
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