Literature DB >> 6661227

FAB-mapping of recombinant-DNA protein products.

H R Morris, M Panico, G W Taylor.   

Abstract

A new method is described for screening and establishing the primary structure of proteins. The procedure is both rapid and sensitive and allows study of the C-terminus of the protein with equal facility to the N-terminus. This new strategy which is illustrated here for the polypeptide hormone Insulin, has obvious applications in recombinant DNA biotechnology research, in post-translational modification and site-directed mutagenesis studies, or any other aspect of modern protein chemistry requiring accurate definition of primary structure.

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Year:  1983        PMID： 6661227     DOI： 10.1016/0006-291x(83)91575-9

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  15 in total

1.  Production and characterization of human gamma interferon from Escherichia coli.

Authors:  L Perez; J Vega; C Chuay; A Menendez; R Ubieta; M Montero; G Padron; A Silva; C Santizo; V Besada
Journal:  Appl Microbiol Biotechnol       Date:  1990-07       Impact factor: 4.813

2.  Peptide sequencing by using a combination of partial acid hydrolysis and fast-atom-bombardment mass spectrometry.

Authors:  F De Angelis; M Botta; S Ceccarelli; R Nicoletti
Journal:  Biochem J       Date:  1986-06-01       Impact factor: 3.857

3.  Further analysis of the sequence of the S1 subunit of pertussis toxin.

Authors:  M Pizza; M Bugnoli; P Pucci; R Siciliano; G Marino; R Rappuoli
Journal:  Infect Immun       Date:  1991-03       Impact factor: 3.441

Review 4.  Fast atom bombardment mass spectrometric characterization of peptides.

Authors:  P R Das; B N Pramanik
Journal:  Mol Biotechnol       Date:  1998-04       Impact factor: 2.695

Review 5.  Fast atom bombardment mass spectrometry and its application to the analysis of some peptides and proteins.

Authors:  M E Hemling
Journal:  Pharm Res       Date:  1987-02       Impact factor: 4.200

6.  Mass spectrometric charting of bovine posterior/intermediate pituitary peptides.

Authors:  G J Feistner; P Højrup; C J Evans; D F Barofsky; K F Faull; P Roepstorff
Journal:  Proc Natl Acad Sci U S A       Date:  1989-08       Impact factor: 11.205

7.  Protein sequencing by tandem mass spectrometry.

Authors:  D F Hunt; J R Yates; J Shabanowitz; S Winston; C R Hauer
Journal:  Proc Natl Acad Sci U S A       Date:  1986-09       Impact factor: 11.205

8.  Peptide sequencing by fast atom bombardment mass spectrometry: acid hydrolysis or tandem mass spectrometry?

Authors:  F de Angelis; M Botta; R Nicoletti
Journal:  Biochem J       Date:  1987-07-15       Impact factor: 3.857

9.  Peptide sequencing by magnetic deflection tandem mass spectrometry.

Authors:  H A Scoble; S A Martin; K Biemann
Journal:  Biochem J       Date:  1987-07-15       Impact factor: 3.857

10.  C-terminal peptide identification by fast atom bombardment mass spectrometry.

Authors:  K Rose; L A Savoy; M G Simona; R E Offord; P Wingfield
Journal:  Biochem J       Date:  1988-02-15       Impact factor: 3.857
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