Literature DB >> 1367470

Production and characterization of human gamma interferon from Escherichia coli.

L Perez1, J Vega, C Chuay, A Menendez, R Ubieta, M Montero, G Padron, A Silva, C Santizo, V Besada.   

Abstract

The production of human gamma interferon as intracellular inclusion bodies in Escherichia coli, which simplified the purification process, is described. An expression plasmid carrying lipoprotein and the tryptophane promoters in tandem was used. Preparation of highly pure interferon was achieved using high resolution chromatography after denaturation and renaturation steps. Structural characteristics of this protein were verified by mass spectrometric analysis. Additional control tests have shown the suitability of the final product for clinical purposes.

Entities:  

Mesh：

Substances：

Year:  1990        PMID： 1367470     DOI： 10.1007/bf00176659

Source DB:  PubMed          Journal:  Appl Microbiol Biotechnol        ISSN： 0175-7598            Impact factor:   4.813


  25 in total

1.  A sensitive radioimmunoprecipitation assay for the detection of antibody to recombinant human gamma-interferon: comparison to a bioassay neutralization test.

Authors:  A B Chen; V R Anicetti; T D Klassen; W Berthold; G Zahn; R M Wert; M D Geier; A J Jones
Journal:  J Interferon Res       Date:  1986-06

Review 2.  Structure of interferons.

Authors:  J A Langer; S Pestka
Journal:  Pharmacol Ther       Date:  1985       Impact factor: 12.310

3.  Acid unfolding and self-association of recombinant Escherichia coli derived human interferon gamma.

Authors:  T Arakawa; Y R Hsu; D A Yphantis
Journal:  Biochemistry       Date:  1987-08-25       Impact factor: 3.162

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Structural characterization of human interferon gamma. Heterogeneity of the carboxyl terminus.

Authors:  Y C Pan; A S Stern; P C Familletti; F R Khan; R Chizzonite
Journal:  Eur J Biochem       Date:  1987-07-01

6.  Verification of protein sequence by fast atom bombardment mass spectrometry. Amino acid sequence of protein S, a development-specific protein of Myxococcus xanthus.

Authors:  T Takao; T Hitouji; Y Shimonishi; T Tanabe; S Inouye; M Inouye
Journal:  J Biol Chem       Date:  1984-05-25       Impact factor: 5.157

7.  High-level expression of human interferon gamma in Escherichia coli under control of the pL promoter of bacteriophage lambda.

Authors:  G Simons; E Remaut; B Allet; R Devos; W Fiers
Journal:  Gene       Date:  1984-04       Impact factor: 3.688

8.  Studies on transformation of Escherichia coli with plasmids.

Authors:  D Hanahan
Journal:  J Mol Biol       Date:  1983-06-05       Impact factor: 5.469

9.  Structural studies on acid unfolding and refolding of recombinant human interferon gamma.

Authors:  Y R Hsu; T Arakawa
Journal:  Biochemistry       Date:  1985-12-31       Impact factor: 3.162

10.  Low temperature embedding with Lowicryl resins: two new formulations and some applications.

Authors:  E Carlemalm; W Villiger; J A Hobot; J D Acetarin; E Kellenberger
Journal:  J Microsc       Date:  1985-10       Impact factor: 1.758
View more
  2 in total

1.  Increased yield of high purity recombinant human interferon-gamma utilizing reversed phase column chromatography.

Authors:  Praveen K Reddy; Srinivasa G Reddy; Venkata R Narala; Sangita S Majee; Sudhakar Konda; Sripad Gunwar; Raju C Reddy
Journal:  Protein Expr Purif       Date:  2006-09-06       Impact factor: 1.650

2.  Process development for production of recombinant human interferon-gamma expressed in Escherichia coli.

Authors:  R Khalilzadeh; S A Shojaosadati; N Maghsoudi; J Mohammadian-Mosaabadi; M R Mohammadi; A Bahrami; N Maleksabet; M A Nassiri-Khalilli; M Ebrahimi; H Naderimanesh
Journal:  J Ind Microbiol Biotechnol       Date:  2004-02-19       Impact factor: 3.346
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.