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Literature DB >> 6660501

Concentrated, digestible DNA after hydroxylapatite chromatography with cetylpyridinium bromide precipitation.

Abstract

A method for the direct extraction of the DNA from the unfavorable phosphate eluant of hydroxylapatite chromatography is described. The DNA--reversibly precipitated with the cationic detergent cetylpyridinium bromide--can be subjected to further enzymatic manipulations within minutes. This method is applied to the rapid separation of pBR322 plasmid from the chromosomal DNA.

Entities: Chemical

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Year: 1983 PMID： 6660501 DOI： 10.1016/0003-2697(83)90681-4

Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365

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Cited

14 in total

1. Identification and chromosomal locations of a family of cytochrome P-450 genes for pisatin detoxification in the fungus Nectria haematococca.

Authors: V P Miao; D E Matthews; H D VanEtten
Journal: Mol Gen Genet Date: 1991-04

2. Differential regulation of Na/H antiporter by acid in renal epithelial cells and fibroblasts.

Authors: O W Moe; R T Miller; S Horie; A Cano; P A Preisig; R J Alpern
Journal: J Clin Invest Date: 1991-11 Impact factor: 14.808

3. Rapid renaturation of complementary DNA strands mediated by cationic detergents: a role for high-probability binding domains in enhancing the kinetics of molecular assembly processes.

Authors: B W Pontius; P Berg
Journal: Proc Natl Acad Sci U S A Date: 1991-09-15 Impact factor: 11.205

4. Synthesis, micellization behaviour, DNA/RNA binding and biological studies of a surfactant cobalt(III) complex with dipyrido[3,2-a:2',4'-c](6,7,8,9-tetrahydro)phenazine.

Authors: Karuppiah Nagaraj; Krishnan Senthil Murugan; Pilavadi Thangamuniyandi; Subramanian Sakthinathan
Journal: J Fluoresc Date: 2014-09-27 Impact factor: 2.217

9. Recombinant human hepatitis B virus reverse transcriptase is active in the absence of the nucleocapsid or the viral replication origin, DR1.

Authors: M Seifer; D N Standring
Journal: J Virol Date: 1993-08 Impact factor: 5.103

10. The rat elastase I regulatory element is an enhancer that directs correct cell specificity and developmental onset of expression in transgenic mice.

Authors: R E Hammer; G H Swift; D M Ornitz; C J Quaife; R D Palmiter; R L Brinster; R J MacDonald
Journal: Mol Cell Biol Date: 1987-08 Impact factor: 4.272

Concentrated, digestible DNA after hydroxylapatite chromatography with cetylpyridinium bromide precipitation.

1. Identification and chromosomal locations of a family of cytochrome P-450 genes for pisatin detoxification in the fungus Nectria haematococca.

2. Differential regulation of Na/H antiporter by acid in renal epithelial cells and fibroblasts.

3. Rapid renaturation of complementary DNA strands mediated by cationic detergents: a role for high-probability binding domains in enhancing the kinetics of molecular assembly processes.

4. Synthesis, micellization behaviour, DNA/RNA binding and biological studies of a surfactant cobalt(III) complex with dipyrido[3,2-a:2',4'-c](6,7,8,9-tetrahydro)phenazine.

5. Development of a fungal transformation system based on selection of sequences with promoter activity.

6. Comparison of genomic fragment and clone sequences within a long interspersed repeated sequence of the mouse genome.

7. Common low-density lipoprotein receptor mutations in the French Canadian population.

8. High diversity in DNA of soil bacteria.

9. Recombinant human hepatitis B virus reverse transcriptase is active in the absence of the nucleocapsid or the viral replication origin, DR1.

10. The rat elastase I regulatory element is an enhancer that directs correct cell specificity and developmental onset of expression in transgenic mice.