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Literature DB >> 6656538

Use of antioxidants in the analysis of vitamins A and E in mammalian plasma by high performance liquid chromatography.

Abstract

A simple, sensitive, quantitative method for the simultaneous assay of retinol, alpha-tocopherol and gamma-tocopherol in rat, guinea pig, monkey and human plasma was developed by using high performance liquid chromatography. It was found that antioxidant was required to stabilize the fat-soluble vitamins in the plasma of rats. The effect of several antioxidants on the recovery of fat-soluble vitamins was evaluated. Results showed that 0.125% butylated hydroxytoluene (BHT) in ethanol and 0.025% BHT in heptane yielded recoveries greater than 95% in 0.1 ml plasma.

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Year: 1983 PMID： 6656538

Source DB: PubMed Journal: Lipids ISSN： 0024-4201 Impact factor: 1.880

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Cited

6 in total

Use of antioxidants in the analysis of vitamins A and E in mammalian plasma by high performance liquid chromatography.

1. Laboratory medicine best practice guideline: vitamins a, e and the carotenoids in blood.

2. Plasma alpha- and gamma-tocopherol have different pattern during normal human pregnancy.

3. Distribution of vitamins A and E in blood and liver of rats depleted of vitamin A or vitamin E.

4. Anti-inflammatory effects of vitamin E on experimental lens-induced uveitis.

5. A program of nutritional education in schools reduced the prevalence of iron deficiency in students.

6. A prospective study of the relationship between serum vitamins A and E and risk of breast cancer.