Literature DB >> 6638510

Spectrophotometric quantitation of silver grains eluted from autoradiograms.

M Suissa.   

Abstract

Autoradiograms or fluorograms can be quantified by eluting the silver grains from the developed film with 1 M NaOH and measuring the absorbance of the eluate in a conventional spectrometer. In contrast to direct scanning of the film, the present method is rapid, requires only simple equipment, and permits accurate quantitation of even heavily exposed bands.

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Year:  1983        PMID： 6638510     DOI： 10.1016/0003-2697(83)90117-3

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  85 in total

1.  Exocytic transport vesicles generated in vitro from the trans-Golgi network carry secretory and plasma membrane proteins.

Authors:  J Salamero; E S Sztul; K E Howell
Journal:  Proc Natl Acad Sci U S A       Date:  1990-10       Impact factor: 11.205

2.  Electrochemical potential releases a membrane-bound secretion intermediate of maltose-binding protein in Escherichia coli.

Authors:  B L Geller
Journal:  J Bacteriol       Date:  1990-09       Impact factor: 3.490

3.  Comparison of constitutive and inducible transcriptional enhancement mediated by kappa B-related sequences: modulation of activity in B cells by human T-cell leukemia virus type I tax gene.

Authors:  F Mauxion; C Jamieson; M Yoshida; K Arai; R Sen
Journal:  Proc Natl Acad Sci U S A       Date:  1991-03-15       Impact factor: 11.205

4.  A DNA sequence upstream of the puf operon of Rhodobacter capsulatus is involved in its oxygen-dependent regulation and functions as a protein binding site.

Authors:  G Klug
Journal:  Mol Gen Genet       Date:  1991-04

5.  Developmentally Regulated Expression of the Gene Family for Cytosolic Glutamine Synthetase in Pisum sativum.

Authors:  E L Walker; G M Coruzzi
Journal:  Plant Physiol       Date:  1989-10       Impact factor: 8.340

6.  MluI site-dependent transcriptional regulation of the Candida albicans dUTPase gene.

Authors:  E M McIntosh; J Looser; R H Haynes; R E Pearlman
Journal:  Curr Genet       Date:  1994 Nov-Dec       Impact factor: 3.886

7.  An in vitro system for accurate transcription initiation of chloroplast protein genes.

Authors:  E M Orozco; J E Mullet; N H Chua
Journal:  Nucleic Acids Res       Date:  1985-02-25       Impact factor: 16.971

8.  Nucleotides flanking the promoter sequence influence the transcription of the yeast mitochondrial gene coding for ATPase subunit 9.

Authors:  T K Biswas; G S Getz
Journal:  Proc Natl Acad Sci U S A       Date:  1986-01       Impact factor: 11.205

9.  Rapid induction of phenylalanine ammonia-lyase and chalcone synthase mRNAs during fungus infection of soybean (Glycine max L.) roots or elicitor treatment of soybean cell cultures at the onset of phytoalexin synthesis.

Authors:  H Habereder; G Schröder; J Ebel
Journal:  Planta       Date:  1989-01       Impact factor: 4.116

10.  Age-dependent induction of pyruvate, orthophosphate dikinase in Mesembryanthemum crystallinum L.

Authors:  B Fisslthaler; G Meyer; H J Bohnert; J M Schmitt
Journal:  Planta       Date:  1995       Impact factor: 4.116
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