Literature DB >> 2860636

An in vitro system for accurate transcription initiation of chloroplast protein genes.

E M Orozco, J E Mullet, N H Chua.   

Abstract

We have developed an homologous in vitro system from spinach chloroplasts that correctly initiates transcription of the plastid genes for the large subunit of ribulose-1,5-bisphosphate carboxylase (rbcL) and the beta subunit of the plastid ATPase (atpB). The transcriptionally active extracts from spinach chloroplasts require circular DNA templates for specific initiation. The RNA polymerase activity is insensitive to rifampicin. The extent of transcription in vitro is a function of the extract:template ratio. The efficiency of the rbcL transcription in vitro is more than one transcript per one hundred templates per hour.

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Year:  1985        PMID： 2860636      PMCID： PMC341072          DOI： 10.1093/nar/13.4.1283

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  23 in total

1.  Structures of the genes for the beta and epsilon subunits of spinach chloroplast ATPase indicate a dicistronic mRNA and an overlapping translation stop/start signal.

Authors:  G Zurawski; W Bottomley; P R Whitfeld
Journal:  Proc Natl Acad Sci U S A       Date:  1982-10       Impact factor: 11.205

2.  Differential transcription in vivo and in vitro of two adjacent maize chloroplast genes: The large subunit of ribulosebisphosphate carboxylase and the 2.2-kilobase gene.

Authors:  S O Jolly; L McIntosh; G Link; L Bogorad
Journal:  Proc Natl Acad Sci U S A       Date:  1981-11       Impact factor: 11.205

Review 3.  Regulatory sequences involved in the promotion and termination of RNA transcription.

Authors:  M Rosenberg; D Court
Journal:  Annu Rev Genet       Date:  1979       Impact factor: 16.830

4.  Non-chromosomal antibiotic resistance in bacteria. II. Molecular nature of R-factors isolated from Proteus mirabilis and Escherichia coli.

Authors:  S N Cohen; C A Miller
Journal:  J Mol Biol       Date:  1970-06-28       Impact factor: 5.469

5.  Wheat leaf RNA polymerases. I. Partial purification and characterization of nuclear, chloroplast and soluble DNA-dependent enzymes.

Authors:  G M Polya; A T Jagendorf
Journal:  Arch Biochem Biophys       Date:  1971-10       Impact factor: 4.013

6.  T antigen repression of SV40 early transcription from two promoters.

Authors:  U Hansen; D G Tenen; D M Livingston; P A Sharp
Journal:  Cell       Date:  1981-12       Impact factor: 41.582

7.  The structure of the gene for the large subunit of ribulose 1,5-bisphosphate carboxylase from spinach chloroplast DNA.

Authors:  G Zurawski; B Perrot; W Bottomley; P R Whitfeld
Journal:  Nucleic Acids Res       Date:  1981-07-24       Impact factor: 16.971

8.  The nucleotide sequence, expression, and evolution of one member of a multigene family encoding the small subunit of ribulose-1,5-bisphosphate carboxylase in soybean.

Authors:  S L Berry-Lowe; T D Mc Knight; D M Shah; R B Meagher
Journal:  J Mol Appl Genet       Date:  1982

9.  Selective and accurate initiation of transcription at the Ad2 major late promotor in a soluble system dependent on purified RNA polymerase II and DNA.

Authors:  P A Weil; D S Luse; J Segall; R G Roeder
Journal:  Cell       Date:  1979-10       Impact factor: 41.582

10.  Overlapping divergent genes in the maize chloroplast chromosome and in vitro transcription of the gene for tRNA.

Authors:  Z Schwarz; S O Jolly; A A Steinmetz; L Bogorad
Journal:  Proc Natl Acad Sci U S A       Date:  1981-06       Impact factor: 11.205
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  41 in total

1.  Unique architecture of the plastid ribosomal RNA operon promoter recognized by the multisubunit RNA polymerase in tobacco and other higher plants.

Authors:  Jon Y Suzuki; Priya Sriraman; Zora Svab; Pal Maliga
Journal:  Plant Cell       Date:  2003-01       Impact factor: 11.277

2.  Transcriptional interaction between the promoters of the maize chloroplast genes which encode the beta subunit of ATP synthase and the large subunit of ribulose 1,5-bisphosphate carboxylase.

Authors:  L Hanley-Bowdoin; N H Chua
Journal:  Mol Gen Genet       Date:  1989-01

3.  RNA-protein complexes that form in the spinach chloroplast atpI 5' untranslated region can be divided into two subcomplexes, each comprised of unique cis-elements and trans-factors.

Authors:  Patricia M Merhige; Dawn Both-Kim; Mark D Robida; Margaret J Hollingsworth
Journal:  Curr Genet       Date:  2005-11-04       Impact factor: 3.886

4.  An in vitro transcription termination system to analyze chloroplast promoters: identification of multiple promoters for the spinach atpB gene.

Authors:  L J Chen; S A Rogers; D C Bennett; M C Hu; E M Orozco
Journal:  Curr Genet       Date:  1990-01       Impact factor: 3.886

5.  Protein-DNA interaction within one cloned chloroplast DNA replication origin of Chlamydomonas.

Authors:  Z Q Nie; D Y Chang; M Wu
Journal:  Mol Gen Genet       Date:  1987-09

6.  A primary transcript in spinach chloroplasts that completely lacks a 5' untranslated leader region.

Authors:  D C Bennett; S A Rogers; L J Chen; E M Orozco
Journal:  Plant Mol Biol       Date:  1990-07       Impact factor: 4.076

7.  Pea chloroplast DNA primase: characterization and role in initiation of replication.

Authors:  B L Nielsen; V K Rajasekhar; K K Tewari
Journal:  Plant Mol Biol       Date:  1991-06       Impact factor: 4.076

8.  Sigma-like activity from mustard (Sinapis alba L.) chloroplasts conferring DNA-binding and transcription specificity to E. coli core RNA polymerase.

Authors:  S Bülow; G Link
Journal:  Plant Mol Biol       Date:  1988-07       Impact factor: 4.076

9.  In Vitro Analysis of Light-Induced Transcription in the Wheat psbD/C Gene Cluster Using Plastid Extracts from Dark-Grown and Short-Term-Illuminated Seedlings.

Authors:  T. Wada; Y. Tunoyama; T. Shiina; Y. Toyoshima
Journal:  Plant Physiol       Date:  1994-04       Impact factor: 8.340

10.  Plastid Genes Encoding the Transcription/Translation Apparatus Are Differentially Transcribed Early in Barley (Hordeum vulgare) Chloroplast Development (Evidence for Selective Stabilization of psbA mRNA).

Authors:  B. J. Baumgartner; J. C. Rapp; J. E. Mullet
Journal:  Plant Physiol       Date:  1993-03       Impact factor: 8.340
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