Literature DB >> 6625153

Analysis of modified bases in DNA by stable isotope dilution gas chromatography-mass spectrometry: 5-methylcytosine.

P F Crain, J A McCloskey.   

Abstract

A sensitive assay for 5-methylcytosine in DNA has been developed based on stable isotope dilution gas chromatography-mass spectrometry with selected ion monitoring. 5-[( 2H3]-Methyl)cytosine and [methyl-2H3]thymine have been synthesized as internal standards for analysis of DNA following acid digestion, conversion of pyrimidines to volatile t-butyldimethylsilyl derivatives, and separation in 3 min by gas chromatography. Submicrogram amounts of DNA have been analyzed for 5-methylcytosine content in the range 0.02-1.5 mol%. The estimated limit of quantitative measurement is 0.3 pmol of methylated base in a DNA hydrolysate. The method is compared with other techniques for quantitative measurement of methylated bases in DNA, and 5-methylcytosine levels and precision of analysis for calf thymus, pBR322, and phi X-174 DNAs are reported and compared with literature values. The method can readily be adapted to the accurate high-sensitivity analysis of other methylated bases in DNA.

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Year:  1983        PMID： 6625153     DOI： 10.1016/0003-2697(83)90434-7

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  11 in total

Review 1.  Mass spectrometry of structurally modified DNA.

Authors:  Natalia Tretyakova; Peter W Villalta; Srikanth Kotapati
Journal:  Chem Rev       Date:  2013-02-26       Impact factor: 60.622

2.  Quantitative measurement of dihydrouridine in RNA using isotope dilution liquid chromatography-mass spectrometry (LC/MS).

Authors:  J J Dalluge; T Hashizume; J A McCloskey
Journal:  Nucleic Acids Res       Date:  1996-08-15       Impact factor: 16.971

3.  Differential DNA methylation during the vegetative life cycle of Neurospora crassa.

Authors:  P J Russell; K D Rodland; E M Rachlin; J A McCloskey
Journal:  J Bacteriol       Date:  1987-06       Impact factor: 3.490

4.  Different levels of DNA methylation in yeast and mycelial forms of Candida albicans.

Authors:  P J Russell; J A Welsch; E M Rachlin; J A McCloskey
Journal:  J Bacteriol       Date:  1987-09       Impact factor: 3.490

5.  Multiregional analysis of global 5-methylcytosine and 5-hydroxymethylcytosine throughout the progression of Alzheimer's disease.

Authors:  Elizabeth M Ellison; Erin L Abner; Mark A Lovell
Journal:  J Neurochem       Date:  2017-02       Impact factor: 5.372

6.  The fission yeast gene pmt1+ encodes a DNA methyltransferase homologue.

Authors:  C R Wilkinson; R Bartlett; P Nurse; A P Bird
Journal:  Nucleic Acids Res       Date:  1995-01-25       Impact factor: 16.971

7.  Examination of the DNA substrate selectivity of DNA cytosine methyltransferases using mass tagging.

Authors:  V Rusmintratip; A D Riggs; L C Sowers
Journal:  Nucleic Acids Res       Date:  2000-09-15       Impact factor: 16.971

8.  Structure determination of a new fluorescent tricyclic nucleoside from archaebacterial tRNA.

Authors:  J A McCloskey; P F Crain; C G Edmonds; R Gupta; T Hashizume; D W Phillipson; K O Stetter
Journal:  Nucleic Acids Res       Date:  1987-01-26       Impact factor: 16.971

9.  Novel salvage of queuine from queuosine and absence of queuine synthesis in Chlorella pyrenoidosa and Chlamydomonas reinhardtii.

Authors:  G M Kirtland; T D Morris; P H Moore; J J O'Brian; C G Edmonds; J A McCloskey; J R Katze
Journal:  J Bacteriol       Date:  1988-12       Impact factor: 3.490

10.  Widespread existence of cytosine methylation in yeast DNA measured by gas chromatography/mass spectrometry.

Authors:  Yang Tang; Xiang-Dong Gao; Yinsheng Wang; Bi-Feng Yuan; Yu-Qi Feng
Journal:  Anal Chem       Date:  2012-08-01       Impact factor: 6.986
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