Glucocorticoid receptor in chick erythrocytes.

Glucocorticoid receptor in chick erythrocytes has been characterized. The Scatchard plot analysis of (3H)-dexamethasone binding to red cells showed a single class of binding sites. The apparent Kd of (3H)-dexamethasone binding by whole cell assay was 0.33 nM and the maximum binding capacity was 5.1 fmole/10(7) cells. The apparent Kd of (3H)-dexamethasone binding to cytosol receptor was 0.48 nM. Steroid binding specificity was similar to that reported for leukocytes.