Literature DB >> 6616022

A stable isotope technique for investigating lactate metabolism in humans.

R A Neese, E W Gertz, J A Wisneski, L D Gruenke, J C Craig.   

Abstract

A stable isotope tracer method has been developed for studying lactate metabolism in humans. The method uses lactic acid triple labeled with 13C as the tracer. The stable isotope is infused to attain a level of approximately 1.5% of that of the circulating unlabeled lactate. Following the isolation of lactic acid from the blood, the percentage of triple labeled (13C)lactate is measured using gas chromatography mas spectrometry. We compared this method with tracer methodology using [14C]lactate and found comparable results.

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Year:  1983        PMID： 6616022     DOI： 10.1002/bms.1200100804

Source DB:  PubMed          Journal:  Biomed Mass Spectrom        ISSN： 0306-042X


  4 in total

Review 1.  Metabolomics as a tool for cardiac research.

Authors:  Julian L Griffin; Helen Atherton; John Shockcor; Luigi Atzori
Journal:  Nat Rev Cardiol       Date:  2011-09-20       Impact factor: 32.419

2.  Effects of acute hyperglycemia on myocardial glycolytic activity in humans.

Authors:  J A Wisneski; W C Stanley; R A Neese; E W Gertz
Journal:  J Clin Invest       Date:  1990-05       Impact factor: 14.808

3.  Exogenous nitric oxide reduces glucose transporters translocation and lactate production in ischemic myocardium in vivo.

Authors:  Biao Lei; Ken Matsuo; Volodymyr Labinskyy; Naveen Sharma; Margaret P Chandler; Anna Ahn; Thomas H Hintze; William C Stanley; Fabio A Recchia
Journal:  Proc Natl Acad Sci U S A       Date:  2005-05-03       Impact factor: 11.205

4.  Metabolic fate of extracted glucose in normal human myocardium.

Authors:  J A Wisneski; E W Gertz; R A Neese; L D Gruenke; D L Morris; J C Craig
Journal:  J Clin Invest       Date:  1985-11       Impact factor: 14.808
  4 in total

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