Literature DB >> 6615882

A simple procedure for the synthesis of [32P]phosphoenolpyruvate via the pyruvate kinase exchange reaction at equilibrium.

F F Roossien, J Brink, G T Robillard.   

Abstract

A one step procedure is presented for the preparation of [32P]phosphoenolpyruvate from [gamma-32P]ATP using pyruvate kinase. The reaction is carried out at chemical equilibrium and involves only an exchange of isotope between ATP and phosphoenolpyruvate. The initial phosphoenolpyruvate/ATP ratio in the reaction mixture determines the degree of 32P incorporation into phosphoenolpyruvate when isotopic equilibrium is achieved.

Entities:  

Mesh：

Substances：

Year:  1983        PMID： 6615882     DOI： 10.1016/0304-4165(83)90141-1

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  13 in total

1.  Pyruvate kinase M2 regulates gene transcription by acting as a protein kinase.

Authors:  Xueliang Gao; Haizhen Wang; Jenny J Yang; Xiaowei Liu; Zhi-Ren Liu
Journal:  Mol Cell       Date:  2012-02-02       Impact factor: 17.970

2.  Deuteration of Escherichia coli enzyme I(Ntr) alters its stability.

Authors:  Grzegorz Piszczek; Jennifer C Lee; Nico Tjandra; Chang-Ro Lee; Yeong-Jae Seok; Rodney L Levine; Alan Peterkofsky
Journal:  Arch Biochem Biophys       Date:  2010-12-24       Impact factor: 4.013

3.  Activation of Escherichia coli antiterminator BglG requires its phosphorylation.

Authors:  Fabian M Rothe; Thomas Bahr; Jörg Stülke; Bodo Rak; Boris Görke
Journal:  Proc Natl Acad Sci U S A       Date:  2012-09-10       Impact factor: 11.205

4.  Molecular cloning of the C-terminal domain of Escherichia coli D-mannitol permease: expression, phosphorylation, and complementation with C-terminal permease deletion proteins.

Authors:  D W White; G R Jacobson
Journal:  J Bacteriol       Date:  1990-03       Impact factor: 3.490

5.  The Bacillus subtilis crh gene encodes a HPr-like protein involved in carbon catabolite repression.

Authors:  A Galinier; J Haiech; M C Kilhoffer; M Jaquinod; J Stülke; J Deutscher; I Martin-Verstraete
Journal:  Proc Natl Acad Sci U S A       Date:  1997-08-05       Impact factor: 11.205

6.  Modulation of Escherichia coli adenylyl cyclase activity by catalytic-site mutants of protein IIA(Glc) of the phosphoenolpyruvate:sugar phosphotransferase system.

Authors:  P Reddy; M Kamireddi
Journal:  J Bacteriol       Date:  1998-02       Impact factor: 3.490

7.  Interaction with enzyme IIBMpo (EIIBMpo) and phosphorylation by phosphorylated EIIBMpo exert antagonistic effects on the transcriptional activator ManR of Listeria monocytogenes.

Authors:  Arthur Constant Zébré; Francine Moussan Aké; Magali Ventroux; Rose Koffi-Nevry; Marie-Françoise Noirot-Gros; Josef Deutscher; Eliane Milohanic
Journal:  J Bacteriol       Date:  2015-02-17       Impact factor: 3.490

8.  The HPr protein of the phosphotransferase system links induction and catabolite repression of the Bacillus subtilis levanase operon.

Authors:  J Stülke; I Martin-Verstraete; V Charrier; A Klier; J Deutscher; G Rapoport
Journal:  J Bacteriol       Date:  1995-12       Impact factor: 3.490

9.  Functional characterization of the incomplete phosphotransferase system (PTS) of the intracellular pathogen Brucella melitensis.

Authors:  Marie Dozot; Sandrine Poncet; Cécile Nicolas; Richard Copin; Houda Bouraoui; Alain Mazé; Josef Deutscher; Xavier De Bolle; Jean-Jacques Letesson
Journal:  PLoS One       Date:  2010-09-10       Impact factor: 3.240

10.  The Lactobacillus casei ptsHI47T mutation causes overexpression of a LevR-regulated but RpoN-independent operon encoding a mannose class phosphotransferase system.

Authors:  Alain Mazé; Grégory Boël; Sandrine Poncet; Ivan Mijakovic; Yoann Le Breton; Abdellah Benachour; Vicente Monedero; Josef Deutscher; Axel Hartke
Journal:  J Bacteriol       Date:  2004-07       Impact factor: 3.490
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.