Literature DB >> 6614464

Quantification of coenzymes and related compounds from methanogenic bacteria by high-performance liquid chromatography.

P Van Beelen, W J Geerts, A Pol, G D Vogels.   

Abstract

Quantification of coenzymes and related compounds from methanogens was performed in extracts obtained from whole cells with aqueous ethanol at 80 degrees C. By means of high-performance liquid chromatography the following compounds could be detected and quantified in extracts from Methanobacterium thermoautotrophicum: coenzyme MF430, the prosthetic group of methylcoenzyme M reductase, F560, an oxidation product of this compound, coenzyme F420, F342, methanopterin, and carboxytetrahydromethanopterin, previously known as YFC. Coenzyme MF430, coenzyme F420, and methanopterin could be determined in extracts from Methanosarcina barkeri. Structural differences were noticed between the coenzymes from the methanogenic bacteria studied.

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Year:  1983        PMID: 6614464     DOI: 10.1016/0003-2697(83)90171-9

Source DB:  PubMed          Journal:  Anal Biochem        ISSN: 0003-2697            Impact factor:   3.365


  7 in total

1.  Hydrolysis and reduction of factor 390 by cell extracts of Methanobacterium thermoautotrophicum (strain delta H).

Authors:  S W Kengen; H W von den Hoff; J T Keltjens; C van der Drift; G D Vogels
Journal:  J Bacteriol       Date:  1991-04       Impact factor: 3.490

2.  Relationship between Methanogenic Cofactor Content and Maximum Specific Methanogenic Activity of Anaerobic Granular Sludges.

Authors:  L G Gorris; T M de Kok; B M Kroon; C van der Drift; G D Vogels
Journal:  Appl Environ Microbiol       Date:  1988-05       Impact factor: 4.792

3.  Comparison of methane production rate and coenzyme f(420) content of methanogenic consortia in anaerobic granular sludge.

Authors:  J Dolfing; J W Mulder
Journal:  Appl Environ Microbiol       Date:  1985-05       Impact factor: 4.792

4.  Relationship of Intracellular Coenzyme F(420) Content to Growth and Metabolic Activity of Methanobacterium bryantii and Methanosarcina barkeri.

Authors:  E Heine-Dobbernack; S M Schoberth; H Sahm
Journal:  Appl Environ Microbiol       Date:  1988-02       Impact factor: 4.792

5.  Purification and properties of 5,10-methenyltetrahydromethanopterin cyclohydrolase from Methanosarcina barkeri.

Authors:  B W te Brömmelstroet; C M Hensgens; W J Geerts; J T Keltjens; C van der Drift; G D Vogels
Journal:  J Bacteriol       Date:  1990-02       Impact factor: 3.490

6.  Changes in concentrations of coenzyme F420 analogs during batch growth of Methanosarcina barkeri and Methanosarcina mazei.

Authors:  M W Peck
Journal:  Appl Environ Microbiol       Date:  1989-04       Impact factor: 4.792

7.  7-Methylpterin and 7-methyllumizine: oxidative degradation products of 7-methyl-substituted pteridines in methanogenic bacteria.

Authors:  R H White
Journal:  J Bacteriol       Date:  1985-05       Impact factor: 3.490

  7 in total

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