Literature DB >> 2007552

Hydrolysis and reduction of factor 390 by cell extracts of Methanobacterium thermoautotrophicum (strain delta H).

S W Kengen1, H W von den Hoff, J T Keltjens, C van der Drift, G D Vogels.   

Abstract

Cell extracts of Methanobacterium thermoautotrophicum (strain delta H) were found to perform a hydrogen-dependent reduction of factor 390 (F390), the 8-adenylyl derivative of coenzyme F420. Upon resolution of cell extracts, F390-reducing activity copurified with the coenzyme F420-dependent hydrogenase. This indicates that F390 serves as a substrate of that enzyme. Activity towards F390 was approximately 40-fold lower than that towards coenzyme F420 (0.12 and 5.2 mumol.min-1.mg of protein-1, respectively). In addition, cell extracts catalyzed the hydrolysis of F390 to AMP and coenzyme F420. This hydrolysis required the presence of thiols (6 mM) and much ionic strength (1 M KCl) and was reversibly inhibited by oxygen. The reaction proceeded optimally at pH 8.2 and was Mn dependent. Conditions for F390 hydrolysis in cell extracts are in many respects opposite to those previously described for F390 synthesis.

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Year:  1991        PMID： 2007552      PMCID： PMC207780          DOI： 10.1128/jb.173.7.2283-2288.1991

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  12 in total

1.  Factor 390 chromophores: phosphodiester between AMP or GMP and methanogen factor 420.

Authors:  R P Hausinger; W H Orme-Johnson; C Walsh
Journal:  Biochemistry       Date:  1985-03-26       Impact factor: 3.162

Review 2.  Novel biochemistry of methanogenesis.

Authors:  P E Rouvière; R S Wolfe
Journal:  J Biol Chem       Date:  1988-06-15       Impact factor: 5.157

3.  Methanogen factor 390 formation: species distribution, reversibility and effects of non-oxidative cellular stresses.

Authors:  L M Gloss; R P Hausinger
Journal:  Biofactors       Date:  1988-10       Impact factor: 6.113

4.  Quantification of coenzymes and related compounds from methanogenic bacteria by high-performance liquid chromatography.

Authors:  P Van Beelen; W J Geerts; A Pol; G D Vogels
Journal:  Anal Biochem       Date:  1983-06       Impact factor: 3.365

5.  A rapid, sensitive, and versatile assay for protein using Coomassie brilliant blue G250.

Authors:  J J Sedmak; S E Grossberg
Journal:  Anal Biochem       Date:  1977-05-01       Impact factor: 3.365

6.  The final step in methane formation. Investigations with highly purified methyl-CoM reductase (component C) from Methanobacterium thermoautotrophicum (strain Marburg).

Authors:  J Ellermann; R Hedderich; R Böcher; R K Thauer
Journal:  Eur J Biochem       Date:  1988-03-15

7.  Nickel, cobalt, and molybdenum requirement for growth of Methanobacterium thermoautotrophicum.

Authors:  P Schönheit; J Moll; R K Thauer
Journal:  Arch Microbiol       Date:  1979-10       Impact factor: 2.552

8.  Proposed structure for coenzyme F420 from Methanobacterium.

Authors:  L D Eirich; G D Vogels; R S Wolfe
Journal:  Biochemistry       Date:  1978-10-31       Impact factor: 3.162

9.  Purification and properties of an 8-hydroxy-5-deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum.

Authors:  F S Jacobson; L Daniels; J A Fox; C T Walsh; W H Orme-Johnson
Journal:  J Biol Chem       Date:  1982-04-10       Impact factor: 5.157

10.  Reversible conversion of coenzyme F420 to the 8-OH-AMP and 8-OH-GMP esters, F390-A and F390-G, on oxygen exposure and reestablishment of anaerobiosis in Methanobacterium thermoautotrophicum.

Authors:  A Kiener; W H Orme-Johnson; C T Walsh
Journal:  Arch Microbiol       Date:  1988       Impact factor: 2.552
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  4 in total

1.  Metabolic regulation in methanogenic archaea during growth on hydrogen and CO2.

Authors:  J T Keltjens; G D Vogels
Journal:  Environ Monit Assess       Date:  1996-09       Impact factor: 2.513

2.  Cellular levels of factor 390 and methanogenic enzymes during growth of Methanobacterium thermoautotrophicum deltaH.

Authors:  P Vermeij; J L Pennings; S M Maassen; J T Keltjens; G D Vogels
Journal:  J Bacteriol       Date:  1997-11       Impact factor: 3.490

3.  Growth phase-dependent transcription of the genes that encode the two methyl coenzyme M reductase isoenzymes and N5-methyltetrahydromethanopterin:coenzyme M methyltransferase in Methanobacterium thermoautotrophicum delta H.

Authors:  T D Pihl; S Sharma; J N Reeve
Journal:  J Bacteriol       Date:  1994-10       Impact factor: 3.490

4.  Coenzyme F390 synthetase from Methanobacterium thermoautotrophicum Marburg belongs to the superfamily of adenylate-forming enzymes.

Authors:  P Vermeij; R J van der Steen; J T Keltjens; G D Vogels; T Leisinger
Journal:  J Bacteriol       Date:  1996-01       Impact factor: 3.490
  4 in total

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