Literature DB >> 6614456

A direct solubilization procedure for the determination of DNA and protein in cultured fibroblast monolayers.

T Sorger, R J Germinario.   

Abstract

A procedure for the direct solubilization of fibroblast monolayers allowing precise determination of DNA and protein content has been developed. This simple procedure is applicable to the analysis of these macromolecules in fibroblast cultures containing small numbers of cells (less than 1 X 10(5].

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Year:  1983        PMID： 6614456     DOI： 10.1016/0003-2697(83)90163-x

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  10 in total

1.  Measurement of cell death in mammalian cells.

Authors:  Brian S Cummings; Rick G Schnellmann
Journal:  Curr Protoc Pharmacol       Date:  2004-09-01

2.  UV absorption as an approximation for cell number in in vitro toxicity testing.

Authors:  M J Chang
Journal:  Bull Environ Contam Toxicol       Date:  1991-08       Impact factor: 2.151

3.  Exogenous thymidine is preferentially incorporated into human cytomegalovirus DNA in infected human fibroblasts.

Authors:  J Morin; S Johann; B O'Hara; Y Gluzman
Journal:  J Virol       Date:  1996-09       Impact factor: 5.103

4.  Tissue-specific hypomethylation of the human c-K-ras gene.

Authors:  J Metter; C Cho
Journal:  Nucleic Acids Res       Date:  1989-09-12       Impact factor: 16.971

5.  Activation of human peripheral blood monocytes by lipoproteins.

Authors:  J L Kelley; M M Rozek; C A Suenram; C J Schwartz
Journal:  Am J Pathol       Date:  1988-02       Impact factor: 4.307

6.  Effects of secretory products of breast cancer cells on osteoblast-like cells.

Authors:  M Lacroix; B Siwek; J J Body
Journal:  Breast Cancer Res Treat       Date:  1996       Impact factor: 4.872

7.  Influence of hypercholesterolemia and cholesterol accumulation on rabbit carrageenan granuloma macrophage activation.

Authors:  J L Kelley; C A Suenram; M M Rozek; C J Schwartz
Journal:  Am J Pathol       Date:  1988-06       Impact factor: 4.307

8.  Evidence for dissociation of gluconeogenesis stimulated by non-esterified fatty acids and changes in fructose 2,6-bisphosphate in cultured rat hepatocytes.

Authors:  J N Clore; J S Stillman; S T Helm; W G Blackard
Journal:  Biochem J       Date:  1992-11-15       Impact factor: 3.857

9.  An improved glyoxylic acid technique for the histochemical localization of catecholamines in brown adipose tissue.

Authors:  M K Cottle; W H Cottle; F Pérusse; L J Bukowiecki
Journal:  Histochem J       Date:  1985-12

10.  Organ culture as a model for investigating the effects of antimetabolites and nucleoside transport inhibitors on rodent colonic mucosa.

Authors:  M Moorghen; P Ince; K J Finney; A J Watson; A L Harris
Journal:  In Vitro Cell Dev Biol       Date:  1991-11
  10 in total

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