Literature DB >> 6608103

Purification to homogeneity and partial characterization of interleukin 2 from a human T-cell leukemia.

A S Stern, Y C Pan, D L Urdal, D Y Mochizuki, S DeChiara, R Blacher, J Wideman, S Gillis.   

Abstract

A method utilizing reversed-phase high-performance liquid chromatography has been developed for the purification to homogeneity of interleukin 2 (IL-2) isolated from a human T-cell leukemia. A final purification of 500,000-fold was obtained with a specific activity of pure IL-2 of 10(9) units/mg. The amino acid analysis of natural IL-2 is strikingly similar to the composition deduced from sequence analysis of a cDNA coding for human IL-2. Protein sequence analysis of CNBr-derived peptides yields data consistent with the sequence proposed from cloned cDNA. The availability of homogeneous IL-2 will allow accurate biological studies of its activity free from the contamination of the numerous lymphokine species that are known to be co-produced with IL-2 during the induction procedure.

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Year:  1984        PMID： 6608103      PMCID： PMC344940          DOI： 10.1073/pnas.81.3.871

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  36 in total

1.  Long term culture of tumour-specific cytotoxic T cells.

Authors:  S Gillis; K A Smith
Journal:  Nature       Date:  1977-07-14       Impact factor: 49.962

2.  Selective in vitro growth of T lymphocytes from normal human bone marrows.

Authors:  D A Morgan; F W Ruscetti; R Gallo
Journal:  Science       Date:  1976-09-10       Impact factor: 47.728

3.  Biochemical relationship of thymocyte mitogenic factor and factors enhancing humoral and cell-mediated immune responses.

Authors:  J J Farrar; P L Simon; W J Koopman; J Fuller-Bonar
Journal:  J Immunol       Date:  1978-10       Impact factor: 5.422

4.  Amino acid analysis with fluorescamine at the picomole level.

Authors:  S Stein; P Böhlen; J Stone; W Dairman; S Udenfriend
Journal:  Arch Biochem Biophys       Date:  1973-03       Impact factor: 4.013

5.  T cell growth factor: parameters of production and a quantitative microassay for activity.

Authors:  S Gillis; M M Ferm; W Ou; K A Smith
Journal:  J Immunol       Date:  1978-06       Impact factor: 5.422

6.  In vitro growth of cytotoxic human lymphocytes. I. Growth of cells sensitized in vitro to alloantigens.

Authors:  J L Strausser; S A Rosenberg
Journal:  J Immunol       Date:  1978-10       Impact factor: 5.422

7.  Biochemical characterization of lymphocyte regulatory molecules. II. Purification of a class of rat and human lymphokines.

Authors:  S Gillis; K A Smith; J Watson
Journal:  J Immunol       Date:  1980-04       Impact factor: 5.422

8.  Continuous proliferation of murine antigen-specific helper T lymphocytes in culture.

Authors:  J Watson
Journal:  J Exp Med       Date:  1979-12-01       Impact factor: 14.307

9.  Monoclonal cytolytic T-cell lines.

Authors:  P E Baker; S Gillis; K A Smith
Journal:  J Exp Med       Date:  1979-01-01       Impact factor: 14.307

10.  Biochemical and biological characterization of lymphocyte regulatory molecules. I. Purification of a class of murine lymphokines.

Authors:  J Watson; S Gillis; J Marbrook; D Mochizuki; K A Smith
Journal:  J Exp Med       Date:  1979-10-01       Impact factor: 14.307
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  18 in total

1.  Production of autostimulatory growth factors by the human carcinoma line, RPMI 2650.

Authors:  B M Carey; M Dooley; R Weedle; M Clynes
Journal:  In Vitro Cell Dev Biol       Date:  1993-02

2.  Interleukin-2-like activity in injured rat brain.

Authors:  M Nieto-Sampedro; K G Chandy
Journal:  Neurochem Res       Date:  1987-08       Impact factor: 3.996

3.  Characterization of human interleukin 2 derived from Escherichia coli.

Authors:  S M Liang; B Allet; K Rose; M Hirschi; C M Liang; D R Thatcher
Journal:  Biochem J       Date:  1985-07-15       Impact factor: 3.857

4.  Autologous mixed lymphocyte reaction in man: XV. Cellular and molecular basis of deficient autologous mixed lymphocyte response in insulin-dependent diabetes mellitus.

Authors:  K G Chandy; A M Charles; A Kershnar; B Buckingham; N Waldeck; S Gupta
Journal:  J Clin Immunol       Date:  1984-11       Impact factor: 8.317

5.  Autologous mixed lymphocyte reaction in man. XIV. Deficiency of the autologous mixed lymphocyte reaction in acquired immune deficiency syndrome (AIDS) and AIDS related complex (ARC). In vitro effect of purified interleukin-1 and interleukin-2.

Authors:  S Gupta; S Gillis; M Thornton; M Goldberg
Journal:  Clin Exp Immunol       Date:  1984-11       Impact factor: 4.330

6.  Antigen-specific primary cytotoxic T lymphocyte (CTL) responses in acquired immune deficiency syndrome (AIDS) and AIDS-related complexes (ARC).

Authors:  B Sharma; S Gupta
Journal:  Clin Exp Immunol       Date:  1985-11       Impact factor: 4.330

7.  Modification and secretion of human interleukin 2 produced in insect cells by a baculovirus expression vector.

Authors:  G E Smith; G Ju; B L Ericson; J Moschera; H W Lahm; R Chizzonite; M D Summers
Journal:  Proc Natl Acad Sci U S A       Date:  1985-12       Impact factor: 11.205

8.  Autologous mixed lymphocyte reaction in man. XIII. Characterization of the T-T autologous mixed lymphocyte reaction.

Authors:  S Gupta; G Chandy; M Thornton; M Goldberg
Journal:  J Clin Immunol       Date:  1985-05       Impact factor: 8.317

9.  Purification and renaturation of recombinant human interleukin-2.

Authors:  M P Weir; J Sparks
Journal:  Biochem J       Date:  1987-07-01       Impact factor: 3.857

10.  Characterization of the high-affinity cell-surface receptor for murine B-cell-stimulating factor 1.

Authors:  L S Park; D Friend; K Grabstein; D L Urdal
Journal:  Proc Natl Acad Sci U S A       Date:  1987-03       Impact factor: 11.205
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