Literature DB >> 6586493

Colony hybridization method for screening in situ of eukaryotic amplified genes.

Y Malpièce, M L Michel, G Carloni.   

Abstract

A new method for autoradiographic screening of amplified genes in cellular clones is described. The main feature of the device is to keep viable cells from each clone, which can subsequently be regrown. The availability of this biochemical screening method allows screening for recombinants harboring unselectable markers as well.

Mesh：

Substances：

Year:  1984        PMID： 6586493     DOI： 10.1007/bf01952397

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  17 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

3.  Screening of cloned recombinant DNA in bacteria by in situ colony hybridization.

Authors:  B Cami; P Kourilsky
Journal:  Nucleic Acids Res       Date:  1978-07       Impact factor: 16.971

4.  Localization of a unique gene by direct hybridization in situ.

Authors:  D S Gerhard; E S Kawasaki; F C Bancroft; P Szabo
Journal:  Proc Natl Acad Sci U S A       Date:  1981-06       Impact factor: 11.205

5.  A membrane-filter technique for the detection of complementary DNA.

Authors:  D T Denhardt
Journal:  Biochem Biophys Res Commun       Date:  1966-06-13       Impact factor: 3.575

6.  Transformation of mammalian cells with an amplifiable dominant-acting gene.

Authors:  M Wigler; M Perucho; D Kurtz; S Dana; A Pellicer; R Axel; S Silverstein
Journal:  Proc Natl Acad Sci U S A       Date:  1980-06       Impact factor: 11.205

7.  Expression of a bacterial gene in mammalian cells.

Authors:  R C Mulligan; P Berg
Journal:  Science       Date:  1980-09-19       Impact factor: 47.728

8.  Integrated and free viral DNA in hamster tumors induced by BK virus.

Authors:  N Chenciner; G Meneguzzi; A Corallini; M P Grossi; P Grassi; G Barbanti-Brodano; G Milanesi
Journal:  Proc Natl Acad Sci U S A       Date:  1980-02       Impact factor: 11.205

9.  Nucleotide sequence of the hepatitis B virus genome (subtype ayw) cloned in E. coli.

Authors:  F Galibert; E Mandart; F Fitoussi; P Tiollais; P Charnay
Journal:  Nature       Date:  1979-10-25       Impact factor: 49.962

10.  Excretion of hepatitis B surface antigen particles from mouse cells transformed with cloned viral DNA.

Authors:  M F Dubois; C Pourcel; S Rousset; C Chany; P Tiollais
Journal:  Proc Natl Acad Sci U S A       Date:  1980-08       Impact factor: 11.205
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