Literature DB >> 6586290

Transformation of human cells by DNA transfection.

B M Sutherland, P V Bennett.   

Abstract

Normal human cells can be transformed to anchorage-independent growth by transfection with DNA from MOLT-4 lymphoblasts, derived from a patient with acute lymphocytic leukemia. Cells were treated with polyethylene glycol 6000, and DNA was administered as a calcium phosphate coprecipitate . The transforming activity of MOLT DNA was inactivated by treatment with DNase or by the restriction enzymes Bgll or BamHl but not by EcoRV . DNA from transfected colonies can transform human recipient cells to anchorage-independent growth; the transforming ability and anchorage independence are maintained stably for at least 30 generations.

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Year:  1984        PMID： 6586290

Source DB:  PubMed          Journal:  Cancer Res        ISSN： 0008-5472            Impact factor:   12.701


  7 in total

1.  Clonal populations of the mouse mammary cell line, COMMA-D, which retain capability of morphogenesis in vivo.

Authors:  K G Danielson; J E Knepper; F S Kittrell; J S Butel; D Medina; E M Durban
Journal:  In Vitro Cell Dev Biol       Date:  1989-06

2.  Enhanced transfection efficiency and improved cell survival after electroporation of G2/M-synchronized cells and treatment with sodium butyrate.

Authors:  S Goldstein; C M Fordis; B H Howard
Journal:  Nucleic Acids Res       Date:  1989-05-25       Impact factor: 16.971

Review 3.  Use of cultured human tissues and cells in carcinogenesis research.

Authors:  E W Gabrielson; C C Harris
Journal:  J Cancer Res Clin Oncol       Date:  1985       Impact factor: 4.553

4.  Human recipient cell for oncogene transfection studies.

Authors:  M A Tainsky; F L Shamanski; D Blair; G Vande Woude
Journal:  Mol Cell Biol       Date:  1987-03       Impact factor: 4.272

5.  Transformation of human cells by DNAs ineffective in transformation of NIH 3T3 cells.

Authors:  B M Sutherland; P V Bennett; A G Freeman; S P Moore; P T Strickland
Journal:  Proc Natl Acad Sci U S A       Date:  1985-04       Impact factor: 11.205

6.  Partially transformed, anchorage-independent human diploid fibroblasts result from overexpression of the c-sis oncogene: mitogenic activity of an apparent monomeric platelet-derived growth factor 2 species.

Authors:  C W Stevens; W H Brondyk; J A Burgess; T H Manoharan; B G Häne; W E Fahl
Journal:  Mol Cell Biol       Date:  1988-05       Impact factor: 4.272

7.  Optimal parameters for the polybrene-induced DNA transfection of diploid human fibroblasts.

Authors:  T L Morgan; V M Maher; J J McCormick
Journal:  In Vitro Cell Dev Biol       Date:  1986-06
  7 in total

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