Literature DB >> 6583208

Colorimetric enzymatic measurement of serum total 3 alpha-hydroxy bile acid concentrations without extraction.

M Y Qureshi, S M Smith, G M Murphy.   

Abstract

A new method for the simpler colorimetric assay of serum total 3 alpha-hydroxy bile acid concentrations without prior extraction has been investigated. The lowest concentration that could be reliably measured was 3 mumol/l and the results obtained were in agreement with those obtained using extraction and the spectrofluorimetric enzymatic assay (y = 1.007x - 1.86, r = 0.99, n = 36). Results for 20 normal sera collected after an overnight fast were 6.2 +/- 5.1 mumol/l (mean +/- SD) and in 20 normal plasma randomly collected were 4.9 +/- 4.2 mumol/l. This new assay is comparable in specificity and sensitivity to the older spectrofluorimetric enzymatic assays and convenient for routine use.

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Year:  1984        PMID： 6583208      PMCID： PMC498707          DOI： 10.1136/jcp.37.3.317

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN： 0021-9746            Impact factor:   3.411


  22 in total

1.  Diagnositic value of serum bile acid estimations in liver disease.

Authors:  S Barnes; G A Gallo; D B Trash; J S Morris
Journal:  J Clin Pathol       Date:  1975-06       Impact factor: 3.411

2.  A simplified procedure for the isolation of bile acids from serum based on a batch extraction with the non-ionic resin--Amberlite XAD-7.

Authors:  S Barnes; A Chitranukroh
Journal:  Ann Clin Biochem       Date:  1977-07       Impact factor: 2.057

3.  A simple and sensitive assay of total serum bile acids.

Authors:  F Mashige; K Imai; T Osuga
Journal:  Clin Chim Acta       Date:  1976-07-01       Impact factor: 3.786

4.  Assessment of the plasma disappearance of cholyl'l14C-glycine as a test of hepatocellular disease.

Authors:  B Thjodleifsson; S Barnes; A Chitranukroh; B H Billing; S Sherlock
Journal:  Gut       Date:  1977-09       Impact factor: 23.059

5.  A fluorimetric and enzymatic method for the estimation of serum total bile acids.

Authors:  G M Murphy; B H Billing; D N Baron
Journal:  J Clin Pathol       Date:  1970-10       Impact factor: 3.411

6.  Assessment of activity in chronic active liver disease. Serum bile acids compared with conventional tests and histology.

Authors:  M G Korman; A F Hofmann; W H Summerskill
Journal:  N Engl J Med       Date:  1974-06-20       Impact factor: 91.245

7.  Improved enzymatic assays for bile acids using resazurin and NADH oxidoreductase from Clostridium kluyveri.

Authors:  S Barnes; J G Spenney
Journal:  Clin Chim Acta       Date:  1980-03-28       Impact factor: 3.786

8.  Serum bile acid analysis: a rapid, direct enzymatic method using dual-beam spectrophotofluorimetry.

Authors:  P A Siskos; P T Cahill; N B Javitt
Journal:  J Lipid Res       Date:  1977-09       Impact factor: 5.922

9.  Quantitative determination of serum bile acids using a purified 3 alpha-hydroxysteroid dehydrogenase.

Authors:  O Fausa
Journal:  Scand J Gastroenterol       Date:  1975       Impact factor: 2.423

10.  Dynamics of the enterohepatic circulation of bile acids. Postprandial serum concentrations of conjugates of cholic acid in health, cholecystectomized patients, and patients with bile acid malabsorption.

Authors:  N F LaRusso; M G Korman; N E Hoffman; A F Hofmann
Journal:  N Engl J Med       Date:  1974-10-03       Impact factor: 91.245
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  2 in total

1.  Cerebral blood flow and plasma free tryptophan in cirrhotics with and without hepatic encephalopathy.

Authors:  R Testa; G Rodriguez; F Arvigo; A Grasso; A Gris; F Nobili; S Marenco; G Rosadini; G Celle
Journal:  Ital J Neurol Sci       Date:  1989-08

2.  Analytical evaluation of three enzymatic assays for measuring total bile acids in plasma using a fully-automated clinical chemistry platform.

Authors:  Elisa Danese; Gian Luca Salvagno; Davide Negrini; Giorgio Brocco; Martina Montagnana; Giuseppe Lippi
Journal:  PLoS One       Date:  2017-06-08       Impact factor: 3.240
  2 in total

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