Literature DB >> 4320681

A fluorimetric and enzymatic method for the estimation of serum total bile acids.

G M Murphy, B H Billing, D N Baron.   

Abstract

The enzymatic technique using 3alpha-hydroxysteroid dehydrogenase for determining bile acids in blood has been modified by measuring the reduced nicotinamide adenine dinucleotide fluorimetrically. The increased sensitivity attained enables the concentration of total bile acids in serum to be estimated using 3 ml for normal subjects and 1 ml for jaundiced patients. The range of normal values in serum was found to be 0-4.7 mumol/litre for males and 1.0-8.2 mumol/litre for females.

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Year:  1970        PMID: 4320681      PMCID: PMC476843          DOI: 10.1136/jcp.23.7.594

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN: 0021-9746            Impact factor:   3.411


  12 in total

1.  Principles of the enzymatic measurement of steroids.

Authors:  B HURLOCK; P TALALAY
Journal:  J Biol Chem       Date:  1957-07       Impact factor: 5.157

2.  The fluorometric measurement of pyridine nucleotides.

Authors:  O H LOWRY; N R ROBERTS; J I KAPPHAHN
Journal:  J Biol Chem       Date:  1957-02       Impact factor: 5.157

3.  Induction and purification of alpha- and beta-hydroxysteroid dehydrogenases.

Authors:  P I MARCUS; P TALALAY
Journal:  J Biol Chem       Date:  1956-02       Impact factor: 5.157

4.  Increased sensitivity in fluorometric determinations of nicotinamide nucleotide coenzymes.

Authors:  W G Green; G F Israelstam
Journal:  Can J Biochem       Date:  1968-04

5.  The quantitative determination of bile salts in bile using thin-layer chromatography and 3 alpha-hydroxysteroid dehydrogenase.

Authors:  L A Turnberg; A Anthony-Mote
Journal:  Clin Chim Acta       Date:  1969-05       Impact factor: 3.786

6.  Determination of individual bile acids in biological fluids by thin-layer chromatography and fluorimetry.

Authors:  D Panveliwalla; B Lewis; I D Wootton; S Tabaqchali
Journal:  J Clin Pathol       Date:  1970-05       Impact factor: 3.411

7.  Ultramicrofluorimetric determination of calcium in plasma.

Authors:  M R Lewin; M R Wills; D N Baron
Journal:  J Clin Pathol       Date:  1969-03       Impact factor: 3.411

8.  Conjugated and unconjugated serum bile acid levels n patients with hepatobiliary diseases.

Authors:  I Makino; S Nakagawa; K Mashimo
Journal:  Gastroenterology       Date:  1969-06       Impact factor: 22.682

9.  Quantitative determination of conjugated bile acids in serum in acute hepatitis.

Authors:  B Frosch; H Wagener
Journal:  Nature       Date:  1967-01-28       Impact factor: 49.962

10.  New solvent systems for thin-layer chromatography of bile acids.

Authors:  J A Gregg
Journal:  J Lipid Res       Date:  1966-07       Impact factor: 5.922

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  54 in total

1.  Aspects of bile acid metabolism in cystic fibrosis.

Authors:  M C Goodchild; G M Murphy; A M Howell; S A Nutter; C M Anderson
Journal:  Arch Dis Child       Date:  1975-10       Impact factor: 3.791

2.  Bile acid excretion after pull-through operation for Hirschsprung's disease.

Authors:  H Gaze; G M Murphy; R Nelson; J J Corkery; C M Anderson
Journal:  Arch Dis Child       Date:  1975-03       Impact factor: 3.791

3.  Diagnositic value of serum bile acid estimations in liver disease.

Authors:  S Barnes; G A Gallo; D B Trash; J S Morris
Journal:  J Clin Pathol       Date:  1975-06       Impact factor: 3.411

4.  New technique for analysing conjugated bile acids in gastric juice.

Authors:  D C Gotley; A P Morgan; M J Cooper
Journal:  J Clin Pathol       Date:  1990-11       Impact factor: 3.411

5.  Quantitative-profiling of bile acids and their conjugates in mouse liver, bile, plasma, and urine using LC-MS/MS.

Authors:  Yazen Alnouti; Iván L Csanaky; Curtis D Klaassen
Journal:  J Chromatogr B Analyt Technol Biomed Life Sci       Date:  2008-09-03       Impact factor: 3.205

6.  Influence of bile salts and lipids on intestinal absorption of griseofulvin in man.

Authors:  R Palma; N Vidon; G Houin; A Pfeiffer; M Rongier; J Barre; J J Bernier
Journal:  Eur J Clin Pharmacol       Date:  1986       Impact factor: 2.953

7.  Serum bile acids in the diagnosis of hepatobiliary disease.

Authors:  C R Pennington; P E Ross; I A Bouchier
Journal:  Gut       Date:  1977-11       Impact factor: 23.059

8.  Defective biliary excretion of copper in Wilson's disease.

Authors:  D J Frommer
Journal:  Gut       Date:  1974-02       Impact factor: 23.059

9.  Physiological factors influencing serum bile acid levels.

Authors:  M Ponz De Leon; G M Murphy; R H Dowling
Journal:  Gut       Date:  1978-01       Impact factor: 23.059

10.  Clinical significance of serum bile acid measurement in liver diseases.

Authors:  T Morita; Y Matsuyama; T Fujimoto; M Higuchi; T Tsujii; Y Matsuoka
Journal:  Gastroenterol Jpn       Date:  1978
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