| Literature DB >> 6582062 |
C Van Dop, M Tsubokawa, H R Bourne, J Ramachandran.
Abstract
Transducin was [32P]ADP-ribosylated by cholera toxin in bovine retinal rod outer segments and then partially purified on omega-amino octyl agarose to remove other ADP-ribosylated proteins. Trypsin digestion of the ADP-ribosylated transducin and further purification using boronate-polyacrylamide beads and high performance liquid chromatography yielded a single radiolabeled tetrapeptide, Ser-Arg-Val-Lys. The ADP-ribose is linked to the guanidinium group of arginine.Entities:
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Year: 1984 PMID: 6582062
Source DB: PubMed Journal: J Biol Chem ISSN: 0021-9258 Impact factor: 5.157