Literature DB >> 6576372

Two-dimensional electrophoresis of plasma polypeptides reveals "high" heterozygosity indices.

B B Rosenblum, J V Neel, S M Hanash.   

Abstract

A series of 62 plasma samples have been examined for genetic variation by the technique of two-dimensional polyacrylamide gel electrophoresis followed by silver-staining of the gels. Twenty polypeptides chosen without respect to variability were considered suitable for scoring. Of the total of 1,240 polypeptides, 29 could not be scored unambiguously. Seventy-five of the remaining 1,211 exhibited the combination of a normal and a variant polypeptide. All variants were present in either the father or the mother of the subject. This index of heterozygosity (6.2 +/- 0.7%) is substantially higher than those reported by others in similar studies of human fibroblasts, lymphocytes, kidney, or brain cells.

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Year:  1983        PMID： 6576372      PMCID： PMC384176          DOI： 10.1073/pnas.80.16.5002

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  23 in total

1.  High resolution two-dimensional electrophoresis of proteins.

Authors:  P H O'Farrell
Journal:  J Biol Chem       Date:  1975-05-25       Impact factor: 5.157

2.  High resolution two-dimensional electrophoresis of human plasma proteins.

Authors:  L Anderson; N G Anderson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

3.  Genetic polymorphism in normal human fibroblasts as analyzed by two-dimensional polyacrylamide gel electrophoresis.

Authors:  K E Walton; D Styer; E I Gruenstein
Journal:  J Biol Chem       Date:  1979-08-25       Impact factor: 5.157

4.  Microheterogeneity of serum transferrin, haptoglobin and alpha 2 HS glycoprotein examined by high resolution two-dimensional electrophoresis.

Authors:  N L Anderson; N G Anderson
Journal:  Biochem Biophys Res Commun       Date:  1979-05-14       Impact factor: 3.575

5.  Protein mapping by combined isoelectric focusing and electrophoresis of mouse tissues. A novel approach to testing for induced point mutations in mammals.

Authors:  J Klose
Journal:  Humangenetik       Date:  1975

6.  An approach to completely automatic comparison of two-dimensional electrophoresis gels.

Authors:  M M Skolnick
Journal:  Clin Chem       Date:  1982-04       Impact factor: 8.327

7.  Computer programs for adapting two-dimensional gels to the study of mutation.

Authors:  M M Skolnick; S R Sternberg; J V Neel
Journal:  Clin Chem       Date:  1982-04       Impact factor: 8.327

8.  Genetic analysis of human lymphocyte proteins by two-dimensional gel electrophoresis: 4. Genetic polymorphism of cytosol 100k polypeptide.

Authors:  H Hamaguchi; M Yamada; M Shibasaki; I Kondo
Journal:  Hum Genet       Date:  1982       Impact factor: 4.132

9.  Human hypoxanthine (guanine) phosphoribosyltransferase: an amino acid substitution in a mutant form of the enzyme isolated from a patient with gout.

Authors:  J M Wilson; G E Tarr; W N Kelley
Journal:  Proc Natl Acad Sci U S A       Date:  1983-02       Impact factor: 11.205

10.  Genetic analysis of human lymphocyte proteins by two-dimensional gel electrophoresis: 3. Frequent occurrence of genetic variants in some abundant polypeptides of PHA-stimulated peripheral blood lymphocytes.

Authors:  H Hamaguchi; M Yamada; M Shibasaki; R Mukai; T Yabe; I Kondo
Journal:  Hum Genet       Date:  1982       Impact factor: 4.132
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  21 in total

Review 1.  The grand challenge to decipher the cancer proteome.

Authors:  Samir Hanash; Ayumu Taguchi
Journal:  Nat Rev Cancer       Date:  2010-09       Impact factor: 60.716

2.  Chromosomal localization of structural genes and regulators in wheat by 2D electrophoresis of ditelosomic lines.

Authors:  C Colas des Francs; H Thiellement
Journal:  Theor Appl Genet       Date:  1985-11       Impact factor: 5.699

3.  Is the polymorphism of protein amounts related to phenotypic variability? A comparison of two-dimensional electrophoresis data with morphological traits in maize.

Authors:  C Damerval; Y Hébert; D de Vienne
Journal:  Theor Appl Genet       Date:  1987-06       Impact factor: 5.699

4.  Association of protein amount polymorphism (PAP) among maize lines with performances of their hybrids.

Authors:  A Leonardi; C Damerval; Y Hébert; A Gallais; D de Vienne
Journal:  Theor Appl Genet       Date:  1991-10       Impact factor: 5.699

5.  Identification of a serum protein polymorphism via two-dimensional electrophoresis. Family and population studies in two genetically isolated groups: North American Hutterites and Australian aborigines.

Authors:  H Harrison; K Miller; C Ober; S Refetoff; M Dick; S Elias
Journal:  Am J Hum Genet       Date:  1991-02       Impact factor: 11.025

Review 6.  Molecular genetics of common diseases.

Authors:  J Scott
Journal:  Br Med J (Clin Res Ed)       Date:  1987-09-26

7.  Average locus differences in mutability related to protein "class": a hypothesis.

Authors:  J V Neel
Journal:  Proc Natl Acad Sci U S A       Date:  1990-03       Impact factor: 11.205

8.  Heterozygosity and ethnic variation in Japanese platelet proteins.

Authors:  J Asakawa; J V Neel; N Takahashi; C Satoh; S Kaneoka; E Nishikori; M Fujita
Journal:  Hum Genet       Date:  1988-01       Impact factor: 4.132

9.  Lineage-related polypeptide markers in acute lymphoblastic leukemia detected by two-dimensional gel electrophoresis.

Authors:  S M Hanash; L J Baier; L McCurry; S A Schwartz
Journal:  Proc Natl Acad Sci U S A       Date:  1986-02       Impact factor: 11.205

10.  Genetic variants detected among 106 lymphocyte polypeptides observed in two-dimensional gels.

Authors:  S M Hanash; L J Baier; D Welch; R Kuick; M Galteau
Journal:  Am J Hum Genet       Date:  1986-09       Impact factor: 11.025
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