Literature DB >> 6575958

Cultivation of human osteosarcoma cell lines in serum-free hormone supplemented medium.

K Y Tsang, H H Fudenberg, D C Sun, G S Pai, L R Bishop, S Sager.   

Abstract

Two human osteosarcoma cell lines (TE-85, LM-1) were adapted to grow in serum-free hydrocortisone supplemented medium. Human osteosarcoma associated antigens were detected both on the cell membrane as well as in the concentrated culture medium. TE-85 cells produced bone specific alkaline phosphatase and LM-1 cells produced both bone specific and placental-like alkaline phosphatases when cultured in this medium. Successful proliferation of human osteosarcoma cell lines in serum-free medium is valuable in human osteosarcoma research.

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Year:  1983        PMID： 6575958     DOI： 10.1007/bf02619599

Source DB:  PubMed          Journal:  In Vitro        ISSN： 0073-5655


  19 in total

1.  Placenta-like alkaline phosphatases from human osteosarcoma cells.

Authors:  I Singh; K Y Tsang; W S Blakemore
Journal:  Cancer Res       Date:  1978-01       Impact factor: 12.701

2.  Characteristics of heterologous membrane antigen on cultured human cells.

Authors:  R F Irie; K Irie; D L Morton
Journal:  J Natl Cancer Inst       Date:  1974-12       Impact factor: 13.506

3.  Cultivation in vitro of cells derived from a human osteosarcoma.

Authors:  R M McAllister; M B Gardner; A E Greene; C Bradt; W W Nichols; B H Landing
Journal:  Cancer       Date:  1971-02       Impact factor: 6.860

4.  Production of melanoma-associated antigen(s) by a defined malignant melanoma cell strain grown in chemically defined medium.

Authors:  D O Chee; A W Boddie; J A Roth; E C Holmes; D L Morton
Journal:  Cancer Res       Date:  1976-04       Impact factor: 12.701

5.  Isolation and partial purification of plasma membrane-associated antigens from human osteosarcoma (TE-85) cells in tissue culture.

Authors:  I Singh; K Y Tsang; W S Blakemore
Journal:  Cancer Res       Date:  1976-11       Impact factor: 12.701

6.  Enzymatic susceptibility and spontaneous release of human melanoma tumor-associated antigens.

Authors:  G M Stuhlmiller; H F Seigler
Journal:  J Natl Cancer Inst       Date:  1977-02       Impact factor: 13.506

7.  Detection of tumor-associated antigen in human melanoma cell line supernatants.

Authors:  E A Grimm; H K Silver; J A Roth; D O Chee; R K Gupta; D L Morton
Journal:  Int J Cancer       Date:  1976-05-15       Impact factor: 7.396

8.  Presence of a carcinoma-associated antigen(s) in the spent chemically defined medium of a human colon carcinoma cell line.

Authors:  D O Chee; R K Gupta; D L Morton
Journal:  J Surg Oncol       Date:  1980       Impact factor: 3.454

9.  Characterization of newly established human osteosarcoma cell line, LM-1.

Authors:  K Y Tsang; G S Pai; H H Fudenberg
Journal:  In Vitro       Date:  1981-04

10.  Continuous culture of Reuber hepatoma cells in serum-free arginine-, glutamine- and tyrosine-deprived chemically defined medium.

Authors:  A Niwa; K Yamamoto; K Sorimachi; Y Yasumura
Journal:  In Vitro       Date:  1980-11
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