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Literature DB >> 6572945

Isolation and sequence characterization of a cDNA clone of human antithrombin III.

T Chandra, R Stackhouse, V J Kidd, S L Woo.

Abstract

A human liver cDNA library was constructed by using poly(A)-containing RNA isolated from a human liver biopsy specimen. This library is comprised of 40,000 independent transformants with an average inserted DNA length of 1,200 base pairs. By using the previously cloned baboon antithrombin III cDNA as a specific hybridization probe, greater than 30 human antithrombin III cDNA clones were identified from this library. The clone with the longest DNA insert was selected for sequence analysis. This antithrombin III cDNA clone contains 1,479 base pairs of inserted human DNA and was designated phATIII 113. It contains DNA sequences that code for a signal peptide and the entire mature antithrombin III protein which is comprised of 432 amino acid residues.

Entities: Chemical Gene Species

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Year: 1983 PMID： 6572945 PMCID： PMC393706 DOI： 10.1073/pnas.80.7.1845

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

28 in total

1. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.

Authors: M Grunstein; D S Hogness
Journal: Proc Natl Acad Sci U S A Date: 1975-10 Impact factor: 11.205

2. Inhibition of bovine factor IXa and factor Xabeta by antithrombin III.

Authors: K Kurachi; K Fujikawa; G Schmer; E W Davie
Journal: Biochemistry Date: 1976-01-27 Impact factor: 3.162

3. A new method for sequencing DNA.

Authors: A M Maxam; W Gilbert
Journal: Proc Natl Acad Sci U S A Date: 1977-02 Impact factor: 11.205

4. Anticoagulant action of heparin.

Authors: P S Damus; M Hicks; R D Rosenberg
Journal: Nature Date: 1973-12-07 Impact factor: 49.962

5. Activation of human factor XI (plasma thromboplastin antecedent) by factor XIIa (activated Hageman factor).

Authors: K Kurachi; E W Davie
Journal: Biochemistry Date: 1977-12-27 Impact factor: 3.162

6. The ovalbumin gene. In vitro enzymatic synthesis and characterization.

Authors: J J Monahan; L A McReynolds; B W O'Malley
Journal: J Biol Chem Date: 1976-12-10 Impact factor: 5.157

7. The synthesis and properties of the complete complementary DNA transcript of ovalbumin mRNA.

Authors: J J Monahan; S E Harris; S L Woo; D L Robberson; B W O'Malley
Journal: Biochemistry Date: 1976-01-13 Impact factor: 3.162

8. Physical and chemical characterization of purified ovalbumin messenger RNA.

Authors: S L Woo; J M Rosen; C D Liarakos; Y C Choi; H Busch; A R Means
Journal: J Biol Chem Date: 1975-09-10 Impact factor: 5.157

9. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.

Authors: H Aviv; P Leder
Journal: Proc Natl Acad Sci U S A Date: 1972-06 Impact factor: 11.205

10. The effect of antithrombin III on the activity of the coagulation factors VII, IX and X.

Authors: B Osterud; M Miller-Andersson; U Abildgaard; H Prydz
Journal: Thromb Haemost Date: 1976-04-30 Impact factor: 5.249

29 in total

1. Complete amino acid sequence of human thyroxine-binding globulin deduced from cloned DNA: close homology to the serine antiproteases.

Authors: I L Flink; T J Bailey; T A Gustafson; B E Markham; E Morkin
Journal: Proc Natl Acad Sci U S A Date: 1986-10 Impact factor: 11.205

2. Ligand-dependent enhancement of human antithrombin gene expression by retinoid X receptor alpha and thyroid hormone receptor beta.

Authors: R W Niessen; F Rezaee; P H Reitsma; M Peters; J J de Vijlder; A Sturk
Journal: Biochem J Date: 1996-08-15 Impact factor: 3.857

Isolation and sequence characterization of a cDNA clone of human antithrombin III.

1. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene.

2. Inhibition of bovine factor IXa and factor Xabeta by antithrombin III.

3. A new method for sequencing DNA.

4. Anticoagulant action of heparin.

5. Activation of human factor XI (plasma thromboplastin antecedent) by factor XIIa (activated Hageman factor).

6. The ovalbumin gene. In vitro enzymatic synthesis and characterization.

7. The synthesis and properties of the complete complementary DNA transcript of ovalbumin mRNA.

8. Physical and chemical characterization of purified ovalbumin messenger RNA.

9. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.

10. The effect of antithrombin III on the activity of the coagulation factors VII, IX and X.

1. Complete amino acid sequence of human thyroxine-binding globulin deduced from cloned DNA: close homology to the serine antiproteases.

2. Ligand-dependent enhancement of human antithrombin gene expression by retinoid X receptor alpha and thyroid hormone receptor beta.

3. A method for the simultaneous alignment of three or more amino acid sequences.

4. Molecular cloning and chromosomal localization of human 4-beta-galactosyltransferase.

5. A fragment of antithrombin that binds both heparin and thrombin.

6. Arginine residues are critical for the heparin-cofactor activity of antithrombin III.

7. GT to AT transition at a splice donor site causes skipping of the preceding exon in phenylketonuria.

8. Genetic mapping of the structural gene for antithrombin III to human chromosome 1.

9. The complete amino acid sequence of bovine antithrombin (ATIII).

10. Expression of functionally active human antithrombin III.