Literature DB >> 6572367

Calcium/calmodulin-dependent phosphorylation of vimentin in rat sertoli cells.

W A Spruill, J R Zysk, L L Tres, A L Kierszenbaum.   

Abstract

Ca2+-dependent protein phosphorylation and the role of calmodulin in this process was investigated in subcellular fractions of primary cultures of rat Sertoli cells. Significant Ca2+/calmodulin-dependent protein phosphorylation in Sertoli cells was restricted to the cytosol fraction. The calmodulin dependence of these effects was confirmed by using the calmodulin inhibitor trifluoperazine. One of the Ca2+/calmodulin-dependent phosphoproteins was identified as the intermediate filament protein vimentin, based on the following criteria: (i) migration pattern in two-dimensional polyacrylamide gels, (ii) Ca2+/calmodulin-dependent phosphorylation of a 58-kilodalton protein present in detergent-insoluble intermediate filament protein extract of Sertoli cells, and (iii) peptide mapping of the phosphoprotein. These data support a role for Ca2+/calmodulin-dependent protein phosphorylation in the modulation of Sertoli cell cytoskeletal components.

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Year:  1983        PMID： 6572367      PMCID： PMC393459          DOI： 10.1073/pnas.80.3.760

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  43 in total

1.  Binding of trifluoperazine to the calcium-dependent activator of cyclic nucleotide phosphodiesterase.

Authors:  R M Levin; B Weiss
Journal:  Mol Pharmacol       Date:  1977-07       Impact factor: 4.436

2.  Ca2+-dependent protein phosphorylation system in membranes from various tissues, and its activation by "calcium-dependent regulator".

Authors:  H Schulman; P Greengard
Journal:  Proc Natl Acad Sci U S A       Date:  1978-11       Impact factor: 11.205

3.  High resolution two-dimensional electrophoresis of proteins.

Authors:  P H O'Farrell
Journal:  J Biol Chem       Date:  1975-05-25       Impact factor: 5.157

4.  Studies on adenosine 3',5'-phosphate-binding and adenosine 3',5-phosphate-dependent protein kinase activities associated with subcellular fractions of Chinese hamster ovary cells.

Authors:  A P Li; A W Hsie
Journal:  Biochim Biophys Acta       Date:  1977-11-07

5.  Calcium-dependent protein phosphorylation during secretion by exocytosis in the mast cell.

Authors:  W Sieghart; T C Theoharides; S L Alper; W W Douglas; P Greengard
Journal:  Nature       Date:  1978-09-28       Impact factor: 49.962

6.  Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.

Authors:  D W Cleveland; S G Fischer; M W Kirschner; U K Laemmli
Journal:  J Biol Chem       Date:  1977-02-10       Impact factor: 5.157

7.  Assay of cyclic AMP-dependent protein kinases.

Authors:  J D Corbin; E M Reimann
Journal:  Methods Enzymol       Date:  1974       Impact factor: 1.600

8.  Purification and characterization of cyclic 3',5'-nucleotide phosphodiesterase from bovine brain.

Authors:  W Y Cheung; Y M Lin
Journal:  Methods Enzymol       Date:  1974       Impact factor: 1.600

9.  Multiple cyclic nucleotide phosphodiesterase activities from rat brain.

Authors:  W J Thompson; M M Appleman
Journal:  Biochemistry       Date:  1971-01-19       Impact factor: 3.162

10.  The detertent-resistant cytoskeleton of tissue culture cells includes the nucleus and the microfilament bundles.

Authors:  M Osborn; K Weber
Journal:  Exp Cell Res       Date:  1977-05       Impact factor: 3.905
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  16 in total

1.  The expression of vimentin in epithelial cells from human nasal mucosa.

Authors:  M Kasper; P Stosiek
Journal:  Eur Arch Otorhinolaryngol       Date:  1990       Impact factor: 2.503

Review 2.  Protein phosphorylation in the pancreatic B-cell.

Authors:  D E Harrison; S J Ashcroft; M R Christie; J M Lord
Journal:  Experientia       Date:  1984-10-15

3.  Follicle-stimulating hormone-dependent phosphorylation of vimentin in cultures of rat Sertoli cells.

Authors:  W A Spruill; A L Steiner; L L Tres; A L Kierszenbaum
Journal:  Proc Natl Acad Sci U S A       Date:  1983-02       Impact factor: 11.205

4.  Rat Sertoli cells acquire a beta-adrenergic response during primary culture.

Authors:  A L Kierszenbaum; W A Spruill; M G White; L L Tres; J P Perkins
Journal:  Proc Natl Acad Sci U S A       Date:  1985-04       Impact factor: 11.205

5.  Basal phosphorylation of cyclic AMP-regulated phosphoproteins in intact S49 mouse lymphoma cells.

Authors:  R A Steinberg; Z Kiss
Journal:  Biochem J       Date:  1985-05-01       Impact factor: 3.857

6.  Expression of fetal-type intermediate filaments by 17-day-old rat Sertoli cells cultured on reconstituted basement membrane.

Authors:  F Guillou; C Monet-Kuntz; I Fontaine; J E Flechon
Journal:  Cell Tissue Res       Date:  1990-05       Impact factor: 5.249

7.  Characterization of cyclic AMP-binding proteins in rat sertoli cells using a photoaffinity ligand.

Authors:  W A Spruill; A L Steiner; L L Tres; A L Kierszenbaum
Journal:  Mol Cell Biochem       Date:  1984       Impact factor: 3.396

8.  Phosphorylation of vimentin in mitotically selected cells. In vitro cyclic AMP-independent kinase and calcium-stimulated phosphatase activities.

Authors:  R M Evans
Journal:  J Cell Biol       Date:  1989-01       Impact factor: 10.539

9.  A lens intercellular junction protein, MP26, is a phosphoprotein.

Authors:  K R Johnson; P D Lampe; K C Hur; C F Louis; R G Johnson
Journal:  J Cell Biol       Date:  1986-04       Impact factor: 10.539

10.  Changes in cell shape correlate with collagenase gene expression in rabbit synovial fibroblasts.

Authors:  J Aggeler; S M Frisch; Z Werb
Journal:  J Cell Biol       Date:  1984-05       Impact factor: 10.539
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