Literature DB >> 6526272

Cloning of human adenosine deaminase cDNA and expression in mouse cells.

D Valerio, R S McIvor, S R Williams, M G Duyvesteyn, H van Ormondt, A J van der Eb, D W Martin.   

Abstract

A previously isolated partial cDNA sequence encoding human adenosine deaminase (ADA) was used to probe a cDNA library prepared from human cultured cell mRNA. Clones containing a combined overlapping length of 1462 bp were isolated and sequenced. One of these was found to include the entire ADA coding region. An open reading frame consisting of 363 codons was identified, predicting a polypeptide of Mr 40762. A mammalian expression plasmid was constructed, positioning the ADA coding sequence to be under transcriptional control of the mouse metallothionein promoter. Transfection of cultured mouse L-cells with this plasmid resulted in the acute expression of human ADA enzymatic activity, as assayed by isoelectric focusing.

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Year:  1984        PMID： 6526272     DOI： 10.1016/0378-1119(84)90205-1

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  13 in total

1.  Isolation and characterization of a variant dihydrofolate reductase cDNA from methotrexate-resistant murine L5178Y cells.

Authors:  R S McIvor; C C Simonsen
Journal:  Nucleic Acids Res       Date:  1990-12-11       Impact factor: 16.971

2.  Construction of eukaryotic expression vector pBlacz and its expression both in vitro and in vivo.

Authors:  S Qu; S Yang; Y You; S Liu; S He
Journal:  J Tongji Med Univ       Date:  1996

3.  Homozygosity for a newly identified missense mutation in a patient with very severe combined immunodeficiency due to adenosine deaminase deficiency (ADA-SCID).

Authors:  R Hirschhorn; V Chakravarti; J Puck; S D Douglas
Journal:  Am J Hum Genet       Date:  1991-10       Impact factor: 11.025

4.  Severe combined immune deficiency due to a homozygous 3.2-kb deletion spanning the promoter and first exon of the adenosine deaminase gene.

Authors:  T M Berkvens; E J Gerritsen; M Oldenburg; C Breukel; J T Wijnen; H van Ormondt; J M Vossen; A J van der Eb; P Meera Khan
Journal:  Nucleic Acids Res       Date:  1987-11-25       Impact factor: 16.971

5.  Adenosine deaminase mRNA expression is regulated posttranscriptionally during differentiation of HL-60 cells.

Authors:  T M Berkvens; F Schoute; H van Ormondt; P M Khan; A J van der Eb
Journal:  Nucleic Acids Res       Date:  1987-08-25       Impact factor: 16.971

6.  Adenosine deaminase gene expression is regulated posttranscriptionally in the nucleus.

Authors:  T M Berkvens; F Schoute; H van Ormondt; P Meera Khan; A J van der Eb
Journal:  Nucleic Acids Res       Date:  1988-04-25       Impact factor: 16.971

7.  Somatic mosaicism for a newly identified splice-site mutation in a patient with adenosine deaminase-deficient immunodeficiency and spontaneous clinical recovery.

Authors:  R Hirschhorn; D R Yang; A Israni; M L Huie; D R Ownby
Journal:  Am J Hum Genet       Date:  1994-07       Impact factor: 11.025

8.  Efficient retrovirus-mediated transfer and expression of a human adenosine deaminase gene in diploid skin fibroblasts from an adenosine deaminase-deficient human.

Authors:  T D Palmer; R A Hock; W R Osborne; A D Miller
Journal:  Proc Natl Acad Sci U S A       Date:  1987-02       Impact factor: 11.205

9.  One adenosine deaminase allele in a patient with severe combined immunodeficiency contains a point mutation abolishing enzyme activity.

Authors:  D Valerio; B M Dekker; M G Duyvesteyn; L van der Voorn; T M Berkvens; H van Ormondt; A J van der Eb
Journal:  EMBO J       Date:  1986-01       Impact factor: 11.598

Review 10.  Development of gene therapy: potential in severe combined immunodeficiency due to adenosine deaminase deficiency.

Authors:  Claudia A Montiel-Equihua; Adrian J Thrasher; H Bobby Gaspar
Journal:  Stem Cells Cloning       Date:  2009-12-22
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