Literature DB >> 6517317

High-performance ion-exchange separation of oxidized and reduced nicotinamide adenine dinucleotides.

G A Orr, J S Blanchard.   

Abstract

High-performance anion-exchange chromatography of oxidized and reduced forms of nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP) on a Pharmacia Mono Q anion-exchange column is reported. Microgram quantities of all four nucleotides can be separated at pH 7.7 in approximately 20 min. For preparative purposes, greater than 7 mg of NADH can be purified in a single injection, and the peak fractions have an A260 of greater than 80 OD units with an A260/A340 ratio of 2.25.

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Year:  1984        PMID： 6517317     DOI： 10.1016/0003-2697(84)90544-x

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  9 in total

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2.  Mechanistic studies of morphine dehydrogenase and stabilization against covalent inactivation.

Authors:  E H Walker; C E French; D A Rathbone; N C Bruce
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4.  The association of NADPH with the guanine nucleotide exchange factor from rabbit reticulocytes: a role of pyridine dinucleotides in eukaryotic polypeptide chain initiation.

Authors:  J N Dholakia; T C Mueser; C L Woodley; L J Parkhurst; A J Wahba
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5.  The isoniazid-NAD adduct is a slow, tight-binding inhibitor of InhA, the Mycobacterium tuberculosis enoyl reductase: adduct affinity and drug resistance.

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6.  Superoxide is produced by the reduced flavin in mitochondrial complex I: a single, unified mechanism that applies during both forward and reverse electron transfer.

Authors:  Kenneth R Pryde; Judy Hirst
Journal:  J Biol Chem       Date:  2011-03-10       Impact factor: 5.157

7.  Investigation of NADH binding, hydride transfer, and NAD(+) dissociation during NADH oxidation by mitochondrial complex I using modified nicotinamide nucleotides.

Authors:  James A Birrell; Judy Hirst
Journal:  Biochemistry       Date:  2013-05-30       Impact factor: 3.162

8.  The Mycobacterium tuberculosis Rv2540c DNA sequence encodes a bifunctional chorismate synthase.

Authors:  Fernanda Ely; José E S Nunes; Evelyn K Schroeder; Jeverson Frazzon; Mário S Palma; Diógenes S Santos; Luiz A Basso
Journal:  BMC Biochem       Date:  2008-04-29       Impact factor: 4.059

9.  Production of bio-xylitol from D-xylose by an engineered Pichia pastoris expressing a recombinant xylose reductase did not require any auxiliary substrate as electron donor.

Authors:  Tai Man Louie; Kailin Louie; Samuel DenHartog; Sridhar Gopishetty; Mani Subramanian; Mark Arnold; Shuvendu Das
Journal:  Microb Cell Fact       Date:  2021-02-22       Impact factor: 5.328
  9 in total

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